| 摘要: |
| 目的：本研究我们为了探讨低强度脉冲超声(LIPUS)对人外周血淋巴细胞(HPBL) 增殖和抗肿瘤效应的影响。方法：采用Ficoll梯度离心法分离HPBL；采用强度为0-80 mW/cm2的LIUPS刺激HPBL增殖；CCK-8法检测细胞增殖活性；ELISA法检测抗肿瘤因子(γ干扰素(INF-γ)、白介素2(IL-2)、肿瘤坏死因子α(TNF-α)和粒细胞-巨噬细胞集落刺激因子(GM-CSF))在培养基上清中的表达；采用共培养HPBL与人肺巨细胞癌细胞(95D)检测其抗肿瘤活性。结果：与对照组(0 mW/cm2)相比,LIPUS刺激组(30 mW/cm2)每天刺激10 min,刺激2d后HPBL细胞增殖增强(P<0.01)。抗肿瘤因子刺激组INF-γ(39.66ng/L±8.30)、IL-2(619.84 pg/mL ± 125.94)、TNF-α(461.29 ng/L ± 14.08)和GM-CSF (108.11 ng/L ± 2.07),分别大于对照组(24.40ng/L±6.84)、(279.56 pg/mL ± 128.18)、(417.17 ng/ ± 21.44)和(96.53 ng/L ± 1.56)；共培养实验显示,30 mW/cm2组肺癌杀伤率为51.11%±5.21,对照组为42.86%±0.55。结论：LIPUS刺激后可提高HPBLs的增殖活性和杀伤率。本研究结果可能对LIPUS联合淋巴细胞治疗肿瘤具有较大的治疗潜力和依据。 |
| 关键词: 低强度脉冲超声 人外周血淋巴细胞 细胞因子 细胞增殖 抗肿瘤 |
| DOI： |
| 投稿时间：2019-07-25修订日期：2019-08-12 |
| 基金项目:海南省医药卫生科研项目(2016-SWJW-014-020),海南省自然科学(819QN386) |
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| Enhancement of proliferation and anti-tumor activity of human peripheral blood lymphocytes by low-intensity pulsed ultrasound |
| Huang Denggo,Gao Yuanhui,Cao Hui,Zheng Linlin,Wen Xiaohong,Shufang Zhang |
| (Department of Central Laboratory Affiliated Haikou Hospital of Xiangya School College,Central South University) |
| Abstract: |
| Objective: In this study, we investigated the effects of low intensity pulsed ultrasound (LIPUS) on the proliferation and anti-lung-tumor effect of human peripheral blood lymphocytes (HPBL). Methods: Separation of HPBLs by Ficoll gradient centrifugation. The proliferation of HPBL was stimulated by the intensity of LIUPS between 0 and 80mW/cm2. The proliferation activity of the cells was measured by the CCK-8 assay. The expressions of anti-tumor factors (Interferon-γ (INF- γ), interleukin 2 (IL-2), tumor necrosis factor α (TNF- α) and granulocyte-macrophage colony stimulating factor (GM-CSF) in the supernatant of culture medium were detected by the ELISA assay. The antitumor activity was detected by co-culture of HPBLs and human lung giant cell carcinoma cells (95D). Results: Our study shows that, compared to the control group (0 mW/cm2 ), the proliferation of HPBL cells was enhanced (P < 0.01) after 2 days of stimulation. The intensity of LIPUS is 30 mW/cm2 and duration is 10 minutes a day. With anti-tumor factor, the stimulation group’s INF- γ(39.66ng/L±8.30), IL-2 (619.84 pg/mL ± 125.94), TNF- α(461.29 ng/L± 14.08) and GM-CSF (108.11 ng/L± 2.07).They were higher than the control group (24.40ng/L±6.84), (279.56 pg/mL ± 128.18), (417.17 ng/ ± 21.44) and (96.53 ng/L± 1.56), respectively; Co-culture experiment showed that the killing rate of tumor was 51.11%±5.21 in the 30 mW/cm2 group and 42.86%±0.55 in the control group. Conclusion: The proliferation activity and killing rate of HPBLs were increased after LIPUS stimulation. The results may have great therapeutic potential and basis for the use of LIPUS combined with lymphocytes in the treatment of tumor. |
| Key words: low intensity pulsed ultrasound human peripheral blood lymphocytes cytokines cell proliferation antitumor |
