Objective To investigate the safety and efficacy of ultrasound-guided injection thrombin injection into pseudoaneurysm (PA). Methods The clinical data of 354 patients with iatrogenic pseudoaneurysm who were treated with ultrasound-guided thrombin injection in the Department of Ultrasound, the second affiliated Hospital of Army Medical University from 2015 to 2018 were analyzed retrospectively. Results 21.8% of the patients were diagnosed with complex pseudoaneurysm (77 / 254), and 2 patients were complicated with arteriovenous fistula. The injection dose of thrombin was 50 to 800 UI, with an average of (213.6 ±124.2) UI. 92.9% of patients were successfully treated during the initial thrombin injection. There was no significant difference in the success rate between simple pseudoaneurysm and complex pseudoaneurysm (p>0.05). There is a certain correlation between the size of pseudoaneurysm and the dose of thrombin (p=0.01). No definite evidence of complications was found in clinical and ultrasonic examination after thrombin injection, and no deterioration of blood supply in extremities was found after pseudoaneurysm occlusion. Conclusion Ultrasound-guided thrombin injection into pseudoaneurysm is a safe and highly effective method.