To study the correlation between different ceus parameters and ki-67 protein expression in prostate cancer. Methods A total of 95 male patients diagnosed with prostate cancer by rectal biopsy were collected, and preoperative contrast-enhanced ultrasound was performed. The time-intensity curve of the lesion was analyzed by QLAB software, and quantitative parameters were recorded.The tissues of patients with abnormal enhancement were selected, and the expression of ki-67 protein was detected by immunohistochemistry. The expression of ki-67 protein was divided into negative group, low expression group and high expression group. The correlation between ceus parameters and expression of ki-67 protein was analyzed, and the accuracy of ceus parameters for negative and positive expression of ki-67 was evaluated by using ROC curve. Results AUC, PI, HT, determined by MTT, WIS five contrast-enhanced ultrasound parameters in different ki - 67 protein expression classification comparative differences between groups was statistically significant (P < 0.05), and there was a positive correlation, the correlation coefficient (r value) were 0.657, 0.537, 0.503, 0.407, 0.023, according to the ROC curve display imaging parameter AUC, PI, HT for ki - 67 expression level had higher diagnostic value,When the diagnostic thresholds are >479.44, >7.71, >49.48, the area under the curve is 0.848, 0.793, 0.752, and the AUC has high specificity and sensitivity, respectively 83.64% and 73.24%.Conclusion The expression levels of AUC, PI, HT and ki-67 proteins in the time-intensity curve of ceus are correlated. These parameters can provide a certain basis for the noninvasive evaluation of the proliferation degree of tumor cells in prostate cancer, and can be used as imaging indicators to predict the degree of tumor malignancy.