Objective To explore the diagnostic value of high frequency ultrasound in children with inflammatory bowel disease. Methods To compare the thickness, stratification and surrounding changes of intestinal wall in different parts of children with normal physical examination and inflammatory bowel disease, analyze the sonographic features of high-frequency ultrasound in IBD and summarize the diagnosis points. Results There were significant differences in intestinal wall thickness between normal children and children with inflammatory bowel disease (P < 0.05), and the intestinal wall stratification and surrounding tissues also showed different performances. Conclusion The use of high-frequency ultrasound to detect the thickness of the intestinal wall and its surroundings can provide a powerful reference for the diagnosis of IBD.