AIM: To investigated the diagnostic value of contrast-enhanced ultrasonography in analyzing the blood perfusion status of nude mice pancreatic cancer model. METHODS: Pancreatic cancer orthotopic xenograft tumor model was established. 40 cases of nude mice pancreatic cancer model were stochastically separated into control group (n = 20) and experimental group (n = 20). The experimental group was treated with recombinant human endostatin. The control group was treated with placebo. All animals were underwent contrast-enhanced ultrasound before execution. The peak intensity (PI) and the area under the curve (AUC) were measured by the time intensity curve. Immunohistochemistry was employed to analyze the levels of VEGF and CD34 in pancreatic cancer specimens. Tumor vascular imaging was conducted using the lead oxide-gelatin injection technique. RESULTS: A significant decrease of PI and AUC were seen in experimental group specimens as compared to the control group tissues (P < 0.05). The vascular density was markedly lower in experimental group than that in control group (P < 0.05). The relative levels of VEGF and CD34 were significantly down-regulated in experimental group specimens in comparison with control group tissues (P < 0.05). The PI and AUC were positively correlated with micro vascular density in pancreatic cancer tissues (r = 0.857, P < 0.05; r = 0.762, P < 0.05). The PI and AUC were positively correlated with vascular density per unit volume in pancreatic cancer tissues (r = 0.896, P < 0.05; r = 0.675, P < 0.05). CONCLUSIONS: Contrast-enhanced ultrasound can accurately evaluate the blood perfusion of pancreatic cancer, and the values of PI and AUC are positively related to the vascular density of pancreatic cancer.