Objective To explore the value of contrast-enhanced ultrasound (CEUS) in the differential diagnosis of benign and malignant testicular space-occupying lesions. Methods:The CEUS characteristics of 81 testicular space-occupying lesions examined by CEUS before operation and confirmed by pathologically in our hospital were retrospectively analyzed. The CEUS characteristics of testicular space-occupying lesions were compared to identify benign and malignant, and the multivariate Binary logistic regression analysis of various CEUS indicators were used to predict the degree of malignant risk of testicular space-occupying lesions.Results: There were significant differences in the boundary of enhancement,the speed of contrast agent into testicular space-occupying lesions and enhanced lesion size of testicular space-occupying lesions(P<0.05),There was no significant difference in the degree of enhancement uniformity, enhancement level and clearance rate (P>0.05).three CEUS characteristics associated with malignancy of testicular space-occupying lesions were obtained by binary logistic regression analysis, followed by unclear enhancement boundary, fast wash in, and increased lesion size after enhancement.Conclusions:CEUS provides important value in differentiating benign from malignant testicular space-occupying lesions.