Objective To investigate the influence of ultrasound characteristics of thy- roid nodules on the success rate of ultrasound-guided fine-needle aspiration. Methods Analysis of 235 cases of 251 nodules in our hospital from October 2015 to November 2017.The ultrasonographic features were analyzed: size, location, calcification type, calcification ratio, and solid proportion. According to the classification standard of Bethesda, Bethesda class I is invalid and Bethesda ≥ Ⅱ are valid. Analysis of the influence of correlated Ultrasonic characteristics on FNA. Results Size: group A1 (0.5cm≤d＜1cm)US-FNA had the highest inefficiency of 42.4% (36/85).Compared with the other three groups, the difference was statistically significant(P＜0.05). Depth: group C2(deep level） US-FNA had the highest inefficiency of 41.9% (26/62).Compared with the other two groups , the difference was statistically significant(P＜0.05).Calcification type: group C3 (coarse calcification) US-FNA had the highest inefficiency of (49.1%) (27/55).Compared with the other two groups, the difference was statistically significant (P＜0.05). Calcifi- cation ratio: the inefficiency of US-FNA were 75.0% (15/20) and 80.0% (8/10) higher in D4 group (50%＜C-R≤75%) and E4 group (75%＜C-R).Compared with the other three groups, the difference was statistically significant(P＜0.05).Solid ratio: The inefficiency of US-FNA were 83.3%(5/6)and 80.0%(4/5)higher in A5 group(0%＜S-R≤25%)and B5 group(25%＜S-R≤50%).Compared with the other two groups, the difference was statisti- cally significant(P＜0.05). Conclusion The size of thyroid nodules 0.5cm ≤ d < 1.0 cm, the location is deep, coarse calcification, calcification ratio C-R > 50, solid ratio S-R ≤ 50, to some extent will affect the results of US-FNA unsatisfactory.