| 摘要: |
| 摘 要 目的 探讨EKOS?超声导管联合血栓内注射微泡和尿激酶方法的溶栓治疗效果。方法 采用水听器法测量EKOS?超声导管峰值负压。将90份体外血栓样本随机平均分为超声微泡尿激酶联合组（联合组）、超声尿激酶组（US+UK）、超声微泡组（US+MB）、超声组（US）、尿激酶组（UK）和对照组。计算并比较各组的溶栓率，并对治疗后血栓行HE染色光镜观察。结果 EKOS?超声导管的峰值负压在0.4-6.4 MPa范围内呈无规律动态变化。联合组（41.9±4.5）％与US+UK组（42.0±3.3）％溶栓率接近，均显著高于其余各组（均P <0.05）。这两组治疗后血栓HE染色光镜观察可见与EKOS?超声导管接触处的血栓可见较多崩解，红细胞分布较其余各组更稀疏。US+MB组（28.4±3.3）％与UK组（27.8±3.1）％溶栓率接近，且高于US组（23.9±3.0）％和对照组（17.5±3.7）％（均P <0.05）。结论 EKOS?超声导管可明显提高尿激酶的溶栓率，但在此基础上，增加微泡对溶栓率无明显提高。 |
| 关键词: EKOS?超声导管 微泡 尿激酶 溶栓 |
| DOI： |
| 投稿时间：2019-11-11修订日期：2019-12-30 |
| 基金项目:广州市科技计划项目(201707010083)；湖北省卫计委科研基金面上项目(WJ2018H0078)；武汉市中青年医学骨干人才基金(高顺记) |
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| Microbubble-enhanced sonothrombolysis using endovascular ultrasound catheter with urokinase |
| DONG Rui,ZHU Qiong,FENG Shuang,ZHU Xiansheng,GAO Shunji,LIU Zheng |
| (Department of Ultrasound,the Second Affiliated Hospital of Army Medical University,PLA) |
| Abstract: |
| ABSTRACT Objective To investigate the thrombolytic effect of EKOS? combined with intraclot injection of microbubbles (MB) and urokinase (UK). Methods The peak negative pressure（PNP）of the EKOS? catheter was measured using a hydrophone. Ninety thrombus samples were randomly divided into six groups：ultrasound（US）+MB+UK, US+UK, US+MB, US alone, UK alone and control group. The thrombolysis rate was measured via the mean clot weight loss and compared between groups. Residual clots were histologically observed with hematoxylin-eosin staining and immunofluorescence. Results The PNP of EKOS ? catheter shift irregularly within the range of 0.4-6.4 MPa. The thrombolysis rate of US+MB+UK（41.9±4.5）％ was similar to that of US+UK（42.0±3.3）％, which were significantly higher than that of the other groups ( P <0.05). Histological examination of the clots in US+MB+UK and US+UK showed that the interfaces of clots contacting with EKOS ? catheter were more disintegrable, and the distribution of red blood cells was sparser than other groups. The thrombolysis rate of US+MB (28.4 ±3.3) ％ was close to that of UK alone (27.8±3.1) ％ and significantly higher than that of US alone (23.9 ±3.0) ％ and control group (17.5±3.7) ％ ( P <0.05). Conclusion EKOS? catheter can obviously improve the thrombolysis rate of UK, however, including MB has no significant effect on thrombolysis rate. |
| Key words: EKOS? catheter Microbubbles Urokinase Thrombolysis |
