Objective To evaluate the effect of pulmonary hypertension on right ventricular function in patients with pulmonary hypertension by using three-section echocardiography speckle imaging technology. Methods 68 pulmonary hypertension (PH) patients and 68 healthy controls receiving physical examination with matched basic information were enrolled as subjects for the ultrasonic evaluation of right cardiac function. Speckle tracking imaging technique was applied to measure the right ventricular longitudinal peak systolic strain of three sections: anterior free wall- posterior ventricular septum, middle free wall- middle ventricular septum, posterior free wall- anterior ventricular septum. The overall and 17-segment strain values were obtained and then compared between groups. The correlation between the global strain (GS) of right ventricular and the pulmonary arterial systolic pressure (PASP) was analyzed. Result Three-section speckle-tracking imaging technique proved through multi-angular measurement that the differences of global strain (GS) of right ventricle, basal segment strain of anterior free wall, basal segment strain of free wall, basal segment strain of posterior free wall, middle segment strain of anterior free wall, middle segment strain of free wall, basal segment strain of ventricular septum, basal segment strain of anterior ventricular septum, middle segment strain of ventricular septum, and middle segment strain of anterior ventricular septum between the two groups were statistically significant (P<0.05). Therefore, it was considered that the right ventricular GS and the above-mentioned segment strains in patients of the PH group were reduced from those in patients of the control group. The right ventricular GS presented strong correlation with PASP (P<0.01, r=0.784). Conclusion It is feasible to perform multi-angular analysis on the right cardiac functions of PH patients with three-section speckle-tracking imaging technique. This technology is also an innovative approach of evaluating the overall and regional systolic functions of right ventricle.