ABSTRACT Objective To discuss the accuracy and repeatability of left ventricular structural and functional measurements by two- and three-dimensional contrast-enhanced transthoracic echocardiography (2D cTTE and 3D cTTE). Methods M-mode in parasternal long-axis views were obtained from 12 beagles, and the apical four-chamber, three-chamber and two-chamber views and the three-dimensional full-volume images of the left ventricle were collected with unenhanced and contrast-enhanced transthoracic echocardiography. The intimal display rates in apical four-chamber, three-chamber and two-chamber views of the left ventricular segment were evaluated by two- and three- dimensional unenhanced echocardiography (2D TTE and 3D TTE), 2D cTTE and 3D cTTE. Left ventricular end diastolic longitudinal dimension (LVLD) was measured with 2D TTE and 2D cTTE. Left ventricular end diastolic volume (LVEDV), left ventricular end systolic volume (LVESV) and left ventricular ejection fraction (LVEF) were measured respectively with Teichholz method of M mode, biplane Simpson method of 2D TTE and 2D cTTE. Left ventricular three-dimensional full-volume images were processed and analyzed by TomTec software, LVEDV, LVESV, LVEF were measured respectively with 3D TTE and 3D cTTE. The measurements of pathological specimen were taken as the gold standard, the accuracies of measuring LVLD and LVEDV by different methods were evaluated. All indexes were measured again by the same observer and different observers, and the reproducibility of different methods for measuring different indexes was evaluated. Results ①The intimal display rate of the left ventricular segment was higher with 2D cTTE and 3D cTTE than that with 2D TTE and 3D TTE, 2D cTTE was the best (P<0.05). ②The measurements of LVLD by 2D cTTE were more relevent with the measurements by pathological specimen than that by 2D TTE. (0.851 vs. 0.748). The measurements of LVEDV by M mode, 2D TTE, 2D cTTE, 3D TTE and 3D cTTE correlated with the measurements by pathological specimen (0.612、0.806、0.857、0.728、0.869, respectively). 2D cTTE and 3D cTTE can provide more accurate measurements, 3D cTTE was the most precise method. ③For inter- and intraobservers, the coefficient of variation of LVEDV, LVESV, LVEF with 2D cTTE and 3D cTTE were lower than that with M mode, 2DTTE, 3DTTE. Conclusions Contrast-enhanced transthoracic echocardiography can improve image quality of 2D TTE and 3D TTE with more accurate and repeatable measurements, providing a new method for clinical precise evaluation and dynamic monitoring of left ventricular structure and function.