| 摘要: |
| 目的 探讨超声引导羊膜腔内和胎肺注射肺表面活性物质（PS）预防早产兔肺损伤的效果比较。
方法 将15只新西兰孕兔随机分为胎肺注射PS组（L组）、羊膜腔内注射PS组（A组）及对照组，每组5只。妊娠第27天注药1h后行剖宫产术，观察各组早产兔存活时间，检测支气管肺泡灌洗液中二棕榈胆碱酯磷酸（DPPC）和白细胞介素-6（IL-6）含量，取肺组织行形态学和生化分析。
结果 与对照组相比，L组和A组早产兔存活时间明显延长，DPPC及SP-A含量增加，肺组织细胞凋亡指数和肺损伤病理评分及IL-6含量降低（P＜0.05），II型肺泡上皮细胞板层小体增多、内质网肿胀减少。与A组相比，L组早产兔DPPC、SP-A和IL-6含量增加（P＜0.05），余指标无明显差异。
结论 羊膜腔内和胎肺注射PS均可有效预防早产兔肺损伤，与羊膜腔注射PS相比，胎肺注射等量PS可获得更大效益，有望成为预防早产儿肺损伤的新疗法。 |
| 关键词: 早产 肺损伤 超声引导 肺表面活性物质 |
| DOI： |
| 投稿时间：2019-12-23修订日期：2020-02-01 |
| 基金项目:泉州市科技计划项目(2018C071R)；福建省自然科学(2018J01287)；福建省临床重点专科建设项目资助 |
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| Ultrasound guided intra-amniotic and fetal lung injection of pulmonary surfactant for prevention of lung injury in premature rabbits |
| Laiweicheng,Lvguorong |
| (the Second Affiliated Hospital of Fujian Medical University) |
| Abstract: |
| Objective To compare the effect of ultrasound guided intra-amniotic cavity and fetal lung injection of pulmonary surfactant(PS) to prevent lung injury in premature rabbits. Methods Fifteen pregnant New Zealand rabbits were randomly divided into fetal lung injection PS group (L group), amniotic cavity injection PS group (A group) and control group(each n=5).On the 27th day of pregnancy,rabbit fetuses were removed through cesarean section 1 hour after injection. The survival time of premature rabbits,the content of dipalmitoyl phosphatidylcholine (DPPC) and interleukin-6 (IL-6) in bronchoalveolar lavage fluid ware detected. The lung tissues were taken for morphological and biochemical analysis.Result Compared with control group, the survival time of premature rabbits in L group and A group was significantly prolonged, the content of DPPC and SP-A increased, the apoptosis index of lung tissue and the pathological score of lung injury and IL-6 decreased(P＜0.05). The lamellar bodies of type II alveolar epithelial cells were increased and the swelling of endoplasmic reticulum was decreased. Compared with A group, the content of DPPC、SP-A and IL-6 in preterm rabbits of L group was increased (P ＜0.05), and there was no significant difference in the remaining indicators. Conclusion Both intra-amniotic and fetal lung injection PS can effectively prevent lung injury in premature rabbits. Compared with PS injected into amniotic cavity, PS injection into fetal lung with the same amount can obtain greater benefits, which is expected to be a new treatment to prevent lung injury in premature infants. |
| Key words: preterm birth lung injury ultrasound guided pulmonary surfactant |
