【Abstract】Objective: To investigate the clinical value of ultrasound-guided superficial lymph node biopsy in children. Methods: The clinical features，postoperative pathology and other data of 195 patients underwent ultrasound-guided superficial lymph node biopsy were analyzed retrospectively. Results: 1. Among 195 children, 166 cases of benign diagnosis were made by needle biopsy, of which 1 case was misdiagnosed; 21 cases were malignant; 8 cases were not diagnosed, and 1 case was Hodgkin''s lymphoma.2. Ultrasound-guided biopsy in children with superficial lymph node malignancy: sensitivity is 95.5%, specificity is 100%. The positive predictive value was 100% and the negative predictive value was 99.4%. 3. 186 of 195 patients were diagnosed correctly, and the correct diagnosis rate was 95.4%. The accuracy rate of pathological diagnosis when the material is satisfactory: 99.4% (161/162), and the accuracy rate of diagnosis when the material is not satisfactory: 75.8% (25/33).The correct rate of immunohistochemical pathology was 99.0% (99/100), and the correct rate of immunohistochemical pathology was 91.6% (87/95). The difference was statistically significant (χ2=4.5253, P< 0.05).4. None of the 195 children developed major bleeding or other serious complications. 5. Malignant and benign cases of cervical lymph node lesions involved the most area Ⅱ, accounting for 72.7% (8/11) and 81.6% (71/87), respectively. Conclusion: Ultrasound-guided superficial lymph node biopsy had high specificity and sensitivity, which with the characteristics of easy and safe in operation, low in cost and good tolerance in children. Ultrasound-guided superficial lymph node biopsy had broad application prospects.