Objective To evaluate the effect of anthracycline on right ventricular function in patients with breast cancer by automatic tracking of motion annular displacement. Methods 50 patients with breast cancer using the anthracycline-based chemotherapy were followed up，and routine echocardiography，Two-dimensional speckle tracking technology andautomatic tracking of motion annular displacement were performed before chemotherapy and after 2nd and 4th cycle of chemotherapy.The left ventricular ejection fraction（LVEF），right ventricular fractional area change（FAC），and tricuspid annular plane systolic excursion（TAPSE） were obtained by routine echocardiography. The global longitudinal peak strain of right ventricle （RVGLS） was obtained by two-dimensional speckle tracking technology.The TAD parameters including TAD at the right ventricular free wall （T1），TAD at the interventricular septum （T2），TAD at the midpoint of tricuspid annulus （Tm） and the right ventricular longitudinal shortening （Tm%） were obtained using automatic tracking of motion annular displacement. The differences of the above parameters for each chemotherapy cycle were compared, and the correlation between the TAD parameters and the FAC、TAPSE、RVGLS was analyzed, and the correlation between each parameter of ultrasonic and drug cumulative dose was compared, and we obtained the best value ultrasonic diagnosis parameters by ROC, and further the repeatability of RVGLS and Tm% was tested. Results ①LVEF，FAC and TAPSE were decreased in the 4th cycle compared with the before chemotherapy（all P＜0.05）.RVGLS，T1，T2，Tm and Tm% were lower in the 2nd and 4th cycle compared with the before chemotherapy（all P＜0.05）.②There was a strong correlation between Tm% and RVGLS（r=0.848，0.748，0.795，all P＜0.05），and a weak correlation with RVFAC(r=0.544、0.447、0.347, all P＜0.05) and a weak correlation with TAPSE（r=0.436、0.291、0.305，all P＜0.05). RVGLS，T1，T2，Tm and Tm% were negatively correlated with drug doses（r=0.785，0.852,0.730，0.765，0.862，all P＜0.05), and the correlation was higher than that routine echocardiography.③ROC curve analysis showed that the diagnostic cut off valu of RVGLS is 18.35, the diagnostic sensitivity was 90.7%，specificity was 85.7%, and an area under the curve of 0.94. The diagnostic cut off valu of Tm% is 15.15, the diagnostic sensitivity was 95.3%，specificity was 85.7%, and an area under the curve of 0.93.④Repeatability test showed that the intra-observer ICC values of the mean values of RVGLS and Tm% were 0.891 and 0.937 respectively，and the inter-observer ICC values were 0.850 and 0.890 respectively.Conclusion The technology of aTMAD can be used to identify right ventricular global systolic dysfunction in patients with breast cancer receiving chemotherapy，and Tm% is more convenient than RVGLS，which is repeatable and worthy of clinical promotion.