Objective To investigate the clinical application value of virtual touch tissue imaging quantification (VTIQ) technique in detecting acute liver failure（ALF） and evaluating the effect of preventive medication in rats.Materials and Methods Twenty-four male SD rats were randomly divided into the control group,the model group,and the treatment group,with eight rats in each group. The treatment group was treated by gavage with Medilac-S(100mg·kg-1·d-1) after?dilution, while the control group and the model group were treated with the same volume of warm normal saline for eight days.On the 6th and 7th day of the experiment, the model group and the treatment group were intraperitoneally injected with TAA (200mg·kg-1·d-1). The shear wave velocity(SWV, m/s) in the liver were measured and compared among three groups of rats before and after the establishment of the model，using pathological result as the gold standard to evaluate the degree of liver injury. Results Before modeling, the SWVmean(m/s) of control group, model group and treatment group were 2.43±0.57,2.61±0.42 and 2.71±0.49,the difference had no statistical significance(P＞0.05).After modeling, the SWVmean(m/s) of control group, model group and treatment group were 2.50±0.71,3.56±0.48 and 3.06±0.27.The SWVmean in the model group was higher than that in the control group,the difference had statistical significance(P＜0.05).With cut-off value of 2.83m/s, the AUC, sensitivity and specificity were 0.938, 100% and 75.00% in predicting ALF. The SWVmean in the treatment group was lower than that in the model group,the difference had statistical significance(P＜0.05).Conclusion The VTIQ technique has potential application value in predicting acute liver failure and evaluating the effect of preventive medication.