Objective To evaluate the microcirculatory perfusion function of the thickened and adjacent myocardium by quantitative analysis of myocardial contrast echocardiography(MCE). Methods Among 32 patients with MCE, 16 patients had only thickening of basal segment of interventricular septum (group A1) and middle segment of interventricular septum (group A2); 16 patients in control group had corresponding segments of interventricular septum (Group B1 and group B2). The peak intensity (A), ascending slope (k), peak time (T), A * k and time-intensity curve of myocardial perfusion were compared among the four groups. The ratio of A, K, A * k and T of A1 / A2 and B1 / B2 were analyzed. Results In group A1, A-value, k-value and A* k-value were lower than the other three groups (P < 0.05).The T-value, A* k-value of group A2 were statistically significant with that of group B2 (P < 0.05). The A-value, k-value and A* K ratio of group A1 / A2 were lower than that of group B1 / B2 (P < 0.05), and the time-intensity curve separation of group A1 and group A2 was more distinct than that of group B1 and group B2. Conclusion There were perfusion abnormalities in the segment thickened myocardium and adjacent myocardial microcirculation. Quantitative MCE could early reflect the local myocardial microcirculation dysfunction.