Objective: Using the targeting nanobubbles loaded with galectin-7-siRNA combined with ultrasonic targeted microbubbles destruction (UTMD) to inhibit the expression of galectin-7 and the occurrence of acute rejection after transplantation. Methods: The rat heart transplantation model was constructed and galectin-7-siRNA targeted nanobubbles were prepared. The experiment was divided into four groups: Isograft+PBS treated group, Allograft +PBS+LIFU group，Allograft+NBs treated group and Allograft+NBs+LIFU group. 10 days after transplantation, galectin-7 expression and acute rejection grading in each group of transplanted hearts were compared. Results: the average particle size of MBs were105.84±34.21nm and the average potential was 55.10±2.21 mV. The carrying rate of siGal-7 was 71.2%.10 days after transfection, the expression of galectin-7 protein in the ALLO+NBs+LIFU group was lower than ALLO+PBS+LIFU group and ALLO+NBs treated group (P < 0.05), and the level of acute rejection was significantly lower in the ALLO+MBs+LIFU group than in the ALLO+PBS+LIFU group and ALLO+NBs treated group (P < 0.05). Conclusion: Galectin-7-siRNA targeting nanobubbles can reduce the expression of galectin-7 in the transplanted heart of rats and inhibit the occurrence of acute graft rejection.