| 摘要: |
| 目的：采用超声靶向微泡破坏技术介导galectin-7-siRNA转染,抑制大鼠移植心脏galectin-7表达,达到靶向治疗移植后急性排斥反应的目的。方法：制备携galectin-7-siRNA的ICAM-1靶向微泡；构建大鼠同系移植(ISO)及异系移植(ALLO)心脏模型,并分为4组：ISO+PBS治疗组, ALLO+PBS+LIFU组, ALLO+NBs治疗组及ALLO+NBs+LIFU组,在移植后不同时间点分别给予UTMD治疗,治疗后取材比较各组心脏galectin-7表达及急性排斥反应分级。结果：NBs平均粒径105.84±34.21nm,平均电位55.10±2.21 Mv；siGal-7携带率为 71.2%。ALLO+NBs+LIFU组galectin-7蛋白表达低于ALLO+PBS+LIFU组及ALLO+NBs治疗组(P＜0.05),急性排斥反应分级ALLO+NBs+LIFU组明显低于ALLO+PBS+LIFU组及ALLO+NBs治疗组(P＜0.05)。结论：超声引导下携galectin-7-siRNA靶向纳泡爆破治疗能降低大鼠移植心脏galectin-7表达,抑制急性排斥反应发生。 |
| 关键词: 心脏移植 靶向纳泡 超声 基因治疗 |
| DOI： |
| 投稿时间：2020-01-09修订日期：2020-01-17 |
| 基金项目:国家自然科学基金面上项目(81971626)；国家自然科学基金青年科学(81701707)；黑龙江省自然科学基金杰出青年项目(JQ2019H001)； |
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| Ultrasound Combined with Targeted Nanobubbles Mediated galectin-7-siRNA Transfection inhibiting the acute rejection of rat transplantation hearts |
| wangzhuo,zhaobingbing,lishouqiang,yudandan,zhaochen,jiangshuangquan,lengxiaoping |
| (Department of Radiology, the 2nd Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin, 150001, China) |
| Abstract: |
| Objective: Using the targeting nanobubbles loaded with galectin-7-siRNA combined with ultrasonic targeted microbubbles destruction (UTMD) to inhibit the expression of galectin-7 and the occurrence of acute rejection after transplantation. Methods: The rat heart transplantation model was constructed and galectin-7-siRNA targeted nanobubbles were prepared. The experiment was divided into four groups: Isograft+PBS treated group, Allograft +PBS+LIFU group，Allograft+NBs treated group and Allograft+NBs+LIFU group. 10 days after transplantation, galectin-7 expression and acute rejection grading in each group of transplanted hearts were compared. Results: the average particle size of MBs were105.84±34.21nm and the average potential was 55.10±2.21 mV. The carrying rate of siGal-7 was 71.2%.10 days after transfection, the expression of galectin-7 protein in the ALLO+NBs+LIFU group was lower than ALLO+PBS+LIFU group and ALLO+NBs treated group (P < 0.05), and the level of acute rejection was significantly lower in the ALLO+MBs+LIFU group than in the ALLO+PBS+LIFU group and ALLO+NBs treated group (P < 0.05). Conclusion: Galectin-7-siRNA targeting nanobubbles can reduce the expression of galectin-7 in the transplanted heart of rats and inhibit the occurrence of acute graft rejection. |
| Key words: Heart transplant Targeted Nanobubble Ultrasound Gene Therapy |
