| 摘要: |
| 摘要 目的 观察聚焦超声治疗对SD大鼠外阴慢性单纯性苔藓模型外阴皮肤转化生长因子β及B淋巴细胞瘤-2蛋白表达的影响。方法 建立40只雌性SD大鼠外阴慢性单纯性苔藓模型，随机分为实验组（n = 20）和对照组（n = 20）。实验组大鼠采用聚焦超声治疗，对照组采用聚焦超声假辐照治疗。另取10只正常SD大鼠为空白对照组。聚焦超声治疗后，masson染色鉴定真皮浅层胶原纤维的密度，免疫组化和Western blotting检测转化生长因子β和B淋巴细胞瘤-2在外阴皮肤组织中的表达。比较三个组的组间差异。结果 实验组大鼠中，70%（14/20)的大鼠外阴皮肤恢复正常。对照组大鼠仅10%（2/20）恢复正常，两组间治愈率差异有统计学意义（P＜0.05）。实验组真皮浅层胶原纤维密度明显高于对照组，转化生长因子β蛋白检测在外阴组织中的表达明显低于对照组；而B淋巴细胞瘤-2的蛋白检测则高于对照组。空白对照组蛋白表达趋势均与实验组相同（P＞0.05）。结论 聚焦超声治疗通过影响外阴皮肤组中化生长因子β和B淋巴细胞瘤-2的表达，抑制真皮浅层胶原纤维化，从而达到治疗疾病的目的。 |
| 关键词: 外阴慢性单纯性苔藓 聚焦超声治疗 转化生长因子β3蛋白 B淋巴细胞瘤-2蛋白 |
| DOI： |
| 投稿时间：2020-01-16修订日期：2020-03-05 |
| 基金项目: |
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| Effects of Focused Ultrasound Therapy on the Expression of Transforming Growth Factor β3 and B-cell lymphoma-2 in the vulvar skin of SD Rats with Vulvar Lichen Simplex Chronicus (LSC) |
| Liu Yao,Fan Yijin,秦溢,Li Chengzhi |
| (Chongqing Medical University) |
| Abstract: |
| Objective To observe effects of focused ultrasound therapy on the expression of Transforming Growth Factor β and B-cell lymphoma-2 in the vulvar skin of LSC SD Rats model. Methods Forty female SD rat models were established and randomly divided into test group (n = 20) and control group (n = 20). SD rats in the test group were treated with focused ultrasound, and those in control group received sham treatment. Another 10 normal SD rats were used as the blank control group. After 4 weeks of focused ultrasound treatment, the density of collagen fibers in the superficial dermis was identified by masson staining. The expression of Transforming Growth Factor β and B-cell lymphoma-2 in SD rat’s vulvar tissue was detected by immunohistochemistry and Western blotting. The differences among the three groups were compared. Results In test group, 70 % (14/20) experimental rats returned to normal. In control group, only 10% (2/20) returned to normal. The difference of cure rate between the two groups was statistically significant (P <0.05). The density of collagen fibers in the superficial dermis of the test group was significantly higher than that of control group, and the expression of Transforming Growth Factor β in vulvar tissues was significantly lower than that of the control group, while the expression of B-cell lymphoma-2 was higher than that of the control group. There are the same protein trend between in the test group and in the blank group ( P ＞0.05). Conclusion Focused ultrasound therapy may inhibit the superficial collagen fibrosis of the dermis by affecting the expression of Transforming Growth Factor β and B-cell lymphoma-2 in the vulvar skin tissue, thereby healing the disease. |
| Key words: Vulvar lichen simplex chronicus Focused ultrasound therapy Transforming Growth Factorβ3 B-cell lymphoma-2 |
