Objective To observe effects of focused ultrasound therapy on the expression of Transforming Growth Factor β and B-cell lymphoma-2 in the vulvar skin of LSC SD Rats model. Methods Forty female SD rat models were established and randomly divided into test group (n = 20) and control group (n = 20). SD rats in the test group were treated with focused ultrasound, and those in control group received sham treatment. Another 10 normal SD rats were used as the blank control group. After 4 weeks of focused ultrasound treatment, the density of collagen fibers in the superficial dermis was identified by masson staining. The expression of Transforming Growth Factor β and B-cell lymphoma-2 in SD rat’s vulvar tissue was detected by immunohistochemistry and Western blotting. The differences among the three groups were compared. Results In test group, 70 % (14/20) experimental rats returned to normal. In control group, only 10% (2/20) returned to normal. The difference of cure rate between the two groups was statistically significant (P <0.05). The density of collagen fibers in the superficial dermis of the test group was significantly higher than that of control group, and the expression of Transforming Growth Factor β in vulvar tissues was significantly lower than that of the control group, while the expression of B-cell lymphoma-2 was higher than that of the control group. There are the same protein trend between in the test group and in the blank group ( P ＞0.05). Conclusion Focused ultrasound therapy may inhibit the superficial collagen fibrosis of the dermis by affecting the expression of Transforming Growth Factor β and B-cell lymphoma-2 in the vulvar skin tissue, thereby healing the disease.