Abstract： Objective To evaluate the value of prenatal ultrasound in the diagnosis of fetal split cord malformation. Methods The prenatal ultrasonographic characteristics of 6 cases of fetal split cord malformation in our hospital were analyzed retrospectively, and the ultrasonic features and associated malformations of fetal split cord malformation were summarized. Results In 6 fetuses, 5 cases showed clearly hyperechoic separation in the spinal canal, 1 case showed no echoic cystic structure in the spinal canal; 6 cases showed widened arch spacing and spinal canal expansion in the corresponding position; 5 cases showed the position of the cone of the spinal cord moving down; 3 cases showed that the spinal cord was divided into two parts by compression in the transverse section; 1 case was combined with open spina bifida and myelomeningocele, and 2 cases were combined with multiple hemivertebra and scoliosis. Conclusion The typical prenatal ultrasound features of fetal split cord malformation are hyperechoic in spinal canal and widened space of vertebral arch, often associated with spinal cord below cone position and other spinal deformities. Prenatal ultrasound has important diagnostic and differential diagnostic value.