［］Objective To prepare 10-hydroxycamptothecin(10-HCPT) loaded liquid fluorocarbon target nanoparticles, and to investigate its targeting ability to ovarian carcinoma cells (SKOV-3) and the effect of Ultrasound/CT dual-mode imaging in vitro. Methods PLGA@10-HCPT-PFOB nanoparticles were prepared by double emulsion solvent evaporation process with modified polymeric shell (PLGA-COOH) encapsulating perflurooctyl bromide (PFOB) and 10-HCPT, its general physical properties were observed with optical microscope (OM), scanning electron microscope (SEM) and transmission electron microscope (TEM). The particle size, distribution and Zeta potential were measured by Marvin particle size analyzer. The encapsulation rate and drug load of 10-HCPT were measured by ultraviolet spectrophotometer. The cRGD peptide was conjugated to the nanoparticles by carbodiimide technique. The binding of PLGA@10-HCPT-PFOB to cRGD peptide was detected by Laser Scanning Confocal Microscope (LSCM), and the targeting properties of the nanoparticles were detected in ovarian cancer cells (SKOV-3).The ability of ultrasound imaging and computed tomography(CT) imaging was evaluated in vitro. Results The prepared targeted nanoparticles were yellow suspension, the size distribution of the nanoparticles was uniform under optical microscope, the surface of the targeted nanoparticles was smooth under the scanning electron microscope, the core-shell structure was shown under the transmission electron microscope, and the PFOB and 10-HCPT were wrapped in PLGA, with mean diameter of(322.03±5.34)nm and Zeta potential was(-1.55±0.10)mV. The drug encapsulation and drug loading capacity of cRGD-PLGA@10-HCPT-