Objective To investigate the value of high resolution ultrasonography in demonstrating the anatomical variations of the median vessels and median nerve in the carpal tunnel and it’s clinical significance. Methods A total of 1000 hands of 500 healthy volunteers were included in the study. The location and shape of the bifid median nerve, persistent median artery and persistent median vein were recorded. The diameter of the persistent median artery and persistent median vein in the carpal tunnel were measured. The cross-sectional area of the