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| 携HMME-Gd液态氟碳纳米粒造影剂的制备及体外双模态成像研究 |
| 罗远利,乔斌,曹进,袁勋,谢卓晏,周志益,杨安宇,胡兴华,王志刚,任建丽 |
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| (重庆医科大学附属第二医院超声科;重庆医科大学附属第二医院眼科;中国科学院大学重庆医院（重庆市人民医院）全科医学科;重庆医科大学附属第二医院神经外科;重庆市超声影学研究所) |
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| 摘要: |
| 目的 制备携带血卟啉单甲醚-钆(HMME-Gd)的液态氟碳纳米粒(HMME-Gd-PFPNPs),观察体外US/MRI的成像效果。方法 以磷脂、胆固醇、HMME-Gd和全氟戊烷(PFP)为原料,用薄膜水化法和乳化法制备脂质体纳米粒HMME-Gd-PFPNPs。倒置光学显微镜、透射电子显微镜及激光共聚焦显微镜检测其基本表征；粒径分析仪分析粒径及表面电位；紫外分光光度计分析HMME-Gd的包封率；观察HMME-Gd-PFPNPs体外US/MRI成像的效果。结果 成功制备HMME-Gd-PFPNPs,光镜下为形态规则、大小均一的球形,电镜下为黑色的球形结构。HMME-Gd-PFPNPs平均粒径为(250.27 ± 11.9)nm,电位为(-26.17 ± 0.45)mV,HMME-Gd的包封率为(84.7 ± 0.35)%。在低强度聚焦超声辐照下,体外US成像信号显著增强,与LIFU激发强度成正相关。随HMME-Gd-PFPNPs浓度增加,体外MRI成像效果显著增强。结论 成功制备了携带HMME-Gd的液态氟碳纳米粒,可用于体外US/MRI双模态成像。 |
| 关键词: 纳米粒 造影剂 超声成像 磁共振成像 |
| DOI： |
| 投稿时间：2020-04-20修订日期：2020-04-29 |
| 基金项目:国家自然科学基金(81873901)、重庆市自然科学基金(cstc2019jcyj-zdxmX0020)、宽仁骨干人才项目(KR2019G001)、重庆市渝中区基础研究与前沿探索项目(20190112) |
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| Preparation of HMME-Gd liquid fluorocarbon nanoparticles contrast agent and dual-mode imaging in vitro |
| LUO Yuanli,QIAO Bin,CAO Jin,YUAN Xun,XIE Zhuoyan,ZHOU Zhiyi,YANG Anyu,HU Xinghua,WANG Zhigang,REN Jianli |
| (Chongqing Key Laboratory of Ultrasound Molecular Imaging,Chongqing Institute of Ultrasound Imaging) |
| Abstract: |
| Objective To prepare liquid fluorocarbon nanoparticles carrying HMME-Gd (HMME-Gd-PFPNPs) and observe the effect of ultrasound/MRI imaging capability in vitro. Methods Lipids cholesterol, hematoporphyrin monomethyl ether gadolinium (HMME-Gd) and perfluoropentane (PFP) were used as raw materials to prepare nanoparticles by membrane hydration and emulsification. The general physical properties were measured by optical microscopy, transmission electron microscopy and laser confocal microscope. The size and surface potential were analyzed by particle size analyzer. The encapsulation efficiency of HMME-Gd in HMME-Gd-PFPNPs was determined by UV-vis. The ultrasound (US)/magnetic resonance imaging (MRI) capability of HMME-Gd-PFPNPs was observed in vitro. Results HMME-Gd-PFPNPs were successfully prepared, which were regular in shape and uniform in size. They showed a black spherical structure under transmission electron microscopy. The size of HMME-Gd-PFPNPs was (250.27 ± 11.9) nm, the surface potential was (-26.17 ± 0.45) mV, and the encapsulation efficiency of HMME-Gd was (84.7 ± 0.35) %. As the concentration of HMME-Gd-PFPNPs increased, the MRI signal was significantly enhanced in vitro. Under low intensity focused ultrasound (LIFU) irradiation, the US imaging signal was significantly enhanced in vitro, which was positively correlated with the intensity of LIFU. Conclusion The liquid fluorocarbon nanoparticle contrast agent carrying HMME-Gd was successfully prepared and can be used for US/MRI dualmodal imaging in vitro. |
| Key words: nanoparticles contrast agent ultrasound imaging magnetic resonance imaging |
