Objective To synthesize an IR780 and GOx (glucose oxidase)-based multifunctional nanoplatform, and to examine its active targeting ability and evaluate its capability of photoacoustic (PA) imaging and therapeutic effect of synergistic sonodynamic therapy and starvation therapy. Methods IR780 and GOx-based PLGA nanoplatform was prepared with double emulsion method. The characteristic properties, PA imaging effect, targeting ability and therapeutic outcome of IG@P nanoparticles (IG@P NPs) were observed and performed. Results IG@P NPs showed uniform spherical appearance. The average diameter was (248.1 ± 24.3) nm. The catalytic activity of GOx in NPs was remained. The results of the study showed that IG@P NPs possessed excellent PA imaging effect and active targeting ability. Compared to single therapy, the synergistic SDT and starvation therapy was more fatal to 4T1 cells. Moreover, IG@P NPs can significantly enhance the effect of combination therapy, attributing to the active targeting ability of IR780 which achieve the specific accumulation of NPs in tumor cells.