| 摘要: |
| 目的 制备一种载IR780及葡萄糖氧化酶(GOx)的多功能纳米粒,研究其主动靶向能力,并评估其体外光声成像及声动力(sonodynamic therapy, SDT)/饥饿联合治疗的效果。方法 通过双乳化法制备载有IR780及GOx的聚乳酸-羟基乙酸共聚物(poly (lactic-co-glycolic) acid, PLGA)纳米粒(IG@P 纳米粒) 。检测IG@P 纳米粒的理化特性,观察其体外光声成像的效果,并评估该纳米粒对肿瘤细胞的靶向能力及体外联合治疗效果。结果 制备的IG@P纳米粒呈大小均一的球形,平均粒径(248.1 ± 24.3) nm,且仍保留GOx的酶催化活性。实验结果表明IG@P纳米粒具有良好的光声成像及主动靶向效果。与单一治疗相比,SDT及饥饿治疗联合治疗可以更明显杀伤乳腺癌4T1细胞。且IR780的主动靶向作用,促进纳米粒在肿瘤细胞内的聚集,显著提高了SDT/饥饿治疗的治疗效果。结论 成功制备了具有肿瘤靶向作用的IG@P多功能纳米粒,可用于光声成像,并可增效SDT/饥饿联合治疗。 |
| 关键词: 声动力治疗 饥饿治疗 纳米粒 靶向性 |
| DOI： |
| 投稿时间：2020-07-08修订日期：2020-07-30 |
| 基金项目: |
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| Targeted Anticancer Nanoplatform for Enhanced Synergistic Sonodynamic and Starvation Therapy |
| Ruo Zhang,Liang Zhang,Ju Huang,Zhigang Wang |
| (the Second Affiliated Hospital of Chongqing Medical University) |
| Abstract: |
| Objective To synthesize an IR780 and GOx (glucose oxidase)-based multifunctional nanoplatform, and to examine its active targeting ability and evaluate its capability of photoacoustic (PA) imaging and therapeutic effect of synergistic sonodynamic therapy and starvation therapy. Methods IR780 and GOx-based PLGA nanoplatform was prepared with double emulsion method. The characteristic properties, PA imaging effect, targeting ability and therapeutic outcome of IG@P nanoparticles (IG@P NPs) were observed and performed. Results IG@P NPs showed uniform spherical appearance. The average diameter was (248.1 ± 24.3) nm. The catalytic activity of GOx in NPs was remained. The results of the study showed that IG@P NPs possessed excellent PA imaging effect and active targeting ability. Compared to single therapy, the synergistic SDT and starvation therapy was more fatal to 4T1 cells. Moreover, IG@P NPs can significantly enhance the effect of combination therapy, attributing to the active targeting ability of IR780 which achieve the specific accumulation of NPs in tumor cells. |
| Key words: sonodynamic therapy starvation therapy nanoparticles targeting |
