| 摘要: |
| 目的 制备一种叶酸靶向相变型载羟基喜树碱的纳米粒FA-HCPT-Fe3O4-PFP NDs，考察其理化性能和多模态显像特性。
方法 采用旋转蒸发-超声法制备叶酸靶向相变型载药液态氟碳纳米粒。检测纳米粒的稳定性及相变特性。使用高效液相色谱法检测纳米粒内药物羟基喜树碱的含量，用细胞实验来检测纳米粒寻靶能力。体外实验验证纳米乳液来进行增强超声、光声和磁共振成像。
结果 制备的纳米粒性质稳定，可以发生液气相变。体外实验中，纳米乳液可以明显增强超声、光声及磁共振成像。
结论 本研究所制备的纳米粒具备多模态造影剂的功能，是一种极具潜力的分子探针。 |
| 关键词: 靶向 相变 载药 造影剂 |
| DOI： |
| 投稿时间：2020-07-29修订日期：2021-03-14 |
| 基金项目:] 武汉市卫健委面上重点项目(编号：WX18B11),湖北省自然科学基金面上项目(编号：2018CFB601),湖北省卫健委面上项目(编号：WJ2019M030) |
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| Preparation of Targeted and Phase-shifted Nano-scaled Contrast Agents Loaded with Drug and Detection of#$NBSthe#$NBSBasic#$NBSPerformance |
| Liu jian xin,Liu yang,peng wan hong |
| () |
| Abstract: |
| Objective To prepare phase-shifted folate-modified and HCPT-loaded nanodroplets （termed FA-HCPT-Fe3O4-PFP NDs）and investigate the properties and multimodality imaging in vitro.
Methods The FA-HCPT-Fe3O4-PFP NDs were prepared through the rotary evaporation and sonication process. The stability and phase transition characteristics of the nanodroplets were detected. Drug loading were determined with Reverse-phase high-performance liquid chromatographic system. Targeting efficiency of FA-HCPT-Fe3O4-PFP NDs was validated with cell experiment. FA-HCPT-Fe3O4-PFP NDs nanoemulsions with different concentration were prepared for ultrasound imaging, photoacoustic (PA) imaging and MR imaging.
Results The prepared nanodroplets were stable. Obvious phase-transition was observed of FA-HCPT-Fe3O4-PFP NDs with elevated temperature. FA-HCPT-Fe3O4-PFP NDs nanoemulsions showed obvious PA signal, MRI signal and ultrasound signal in vitro.
Conclusion The successfully fabricated multimodal contrast agent is a molecular robe with great potential. |
| Key words: Targeting, Phase transition, Drug loading, Contrast agents |
