Objective To prepare phase-shifted folate-modified and HCPT-loaded nanodroplets （termed FA-HCPT-Fe3O4-PFP NDs）and investigate the properties and multimodality imaging in vitro. Methods The FA-HCPT-Fe3O4-PFP NDs were prepared through the rotary evaporation and sonication process. The stability and phase transition characteristics of the nanodroplets were detected. Drug loading were determined with Reverse-phase high-performance liquid chromatographic system. Targeting efficiency of FA-HCPT-Fe3O4-PFP NDs was validated with cell experiment. FA-HCPT-Fe3O4-PFP NDs nanoemulsions with different concentration were prepared for ultrasound imaging, photoacoustic (PA) imaging and MR imaging. Results The prepared nanodroplets were stable. Obvious phase-transition was observed of FA-HCPT-Fe3O4-PFP NDs with elevated temperature. FA-HCPT-Fe3O4-PFP NDs nanoemulsions showed obvious PA signal, MRI signal and ultrasound signal in vitro. Conclusion The successfully fabricated multimodal contrast agent is a molecular robe with great potential.