Summary Purpose: To compare the differences in ultrasound and clinicopathological characteristics of triple negative breast cancer (TNBC) patients before and after menopause.Methods: We selected 66 patients with TNBC confirmed by surgery and pathology in our hospital, including 40 premenopausal women (premenopausal group) and 26 postmenopausal women (postmenopausal group). The ultrasound and clinicopathological characteristics of the two groups were retrospectively collected and compared.Results: There were no significant differences in tumor diameter, tumor location, histological grade, Ki-67 expression, and axillary lymph node metastasis between the two groups (all P>0.05). There were statistically significant differences between the two groups in terms of tumor morphology, growth pattern, margin, blood flow grade and BI-RADS classification (P<0.05). There was no significant difference between the two groups in tumor boundary, microcalcification, internal echo, and posterior echo (P>0.05). Compared with the postmenopausal group TNBC, the premenopausal group TNBC showed more regular morphology, horizontal growth, marginal differential lobes, blood flow Adler level 3, and BI-RADS classification into 3-4 categories.Conclusion: Premenopausal and postmenopausal patients with TNBC have some significant differences in ultrasound characteristics, which provide more evidence for the differential diagnosis of TNBC by ultrasound.