by evaluating measurement difference in order to identify the advantages of contrast-enhanced ultrasound (CEUS) in assessing stalk width of gallbladder polyp lesion (GPL) compared to ultrasound (US). Methods: from January 2016 to September 2018, 146 patients with GPL who received cholecystectomy in our hospital were enrolled in this study. All patients had US and CEUS examination before surgery. According to pathological findings, GPL were divided into two groups of cholesterol polyp and adenoma. US features, patient’s age, gender, gallbladder stone, number of GPL, maximum size and stalk width were statistically analyzed between the two groups. Stalk width of cholesterol polyp and adenoma on US and CEUS were analyzed by multiple logistic regression analysis. P value<0.05 was considered statistically significant difference. Results: 104 patients were cholesterol polyp lesion and 42 patients were adenoma, which were proved by final pathological findings. There was no difference in patient’s age, gender, gallbladder stone and number of GPL between two groups (p>0.05), while vascularity and maximum size of GPL were statistically different between two groups (p<0.05). Stalk width on both US and CEUS were statistical differences between cholesterol polyp group and adenoma group (p<0.05). In two groups, stalk width on US was different with that on CEUS (p<0.05). Logistic regression analysis proved that only stalk width on CEUS and vascularity were independent factors related with gallbladder adenoma (p<0.05). Conclusion: CEUS was more suitable for measuring stalk width compared to US. Patients with GPL could get benefit from CEUS for selecting appropriate treatment.