| 摘要: |
| 目的：探讨超声造影定量评估在大鼠肝缺血再灌注损伤(HIRI)动态评估的可行性,观察大鼠HIRI不同再灌注时间超声造影各参数的变化。方法：取SD大鼠49只,随机分为假手术组和HIRI后0.5h、3h、6h、12h、24h、48h组,共7组。建立大鼠HIRI模型,应用超声造影观察各组肝灌注情况,随后使用后处理软件获得峰值强度(PI)、曲线下面积(AUC)、达峰时间(TP)、平均渡越时间(MTT)等参数,并观察其变化情况。取其下腔静脉血检测各样本中谷丙转氨酶(ALT)和天冬氨酸转氨酶(AST)水平。处死大鼠,取其肝组织行病理组织学检查。采用Pearson相关分析超声造影各参数与血清ALT、AST水平的相关性。结果：HIRI 0.5h、3h、6h、12h、24h、48h组PI、AUC水平均低于假手术组,TP、MTT水平均高于假手术组(P＜0.05)。HIRI 0.5h、3h、6h、12h、24h、48h组ALT、AST水平均高于假手术组(P＜0.05)。Pearson相关分析显示,AUC水平与血清ALT、AST水平之间均呈负相关(r=-0.754,r=-0.808,P＜0.001)。TP水平与血清ALT、AST水平之间均呈正相关(r=0.731,r=0.709,P＜0.001)。MTT水平与血清AST水平之间呈正相关(r=0.718,P＜0.001)。结论：超声造影定量分析各参数能够实时准确评估大鼠HIRI微循环的变化来反映其病理变化过程,对HIRI的病情评估具有重要意义。其中AUC是超声造影各参数中评估HIRI的最好指标。 |
| 关键词: 肝缺血再灌注损伤 超声造影 定量分析 动态评估 |
| DOI： |
| 投稿时间：2021-01-19修订日期：2021-02-08 |
| 基金项目: |
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| Experimental Study on Quantitative Evaluation of Contrast-enhanced Ultrasound in Dynamic Evaluation of Hepatic Ischemia-reperfusion Injury in Mice |
| NAN Geli,Ruan Xinyu,Liu Jie,Wang Li |
| () |
| Abstract: |
| Objective: To investigate the feasibility of contrast-enhanced ultrasound quantitative assessment in the dynamic assessment of hepatic ischemia-reperfusion injury (HIRI) in mice, and to observe the changes of various parameters of contrast-enhanced ultrasound in different reperfusion times of HIRI. Methods: 49 SD mice were randomly divided into the sham operation group and the 0.5h, 3h, 6h, 12h, 24h, 48h groups after HIRI, a total of 7 groups. Establishment of HIRI model in mice, use contrast-enhanced ultrasound to observe the liver perfusion in each group, and then use post-processing software to obtain peak intensity (PI), area under the curve (AUC), peak time (TP), mean transit time (MTT), and the changes were observed. The inferior vena cava blood was taken to detect the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in each sample. The mice were sacrificed and their liver tissues were taken for histopathological examination. Pearson correlation analysis was used to analyze the correlation between each parameter of contrast-enhanced ultrasound and serum ALT and AST levels. Results: The PI and AUC levels in the HIRI 0.5h, 3h, 6h, 12h, 24h, 48h group were lower than the sham operation group, and the TP and MTT levels were higher than the sham operation group, and the differences were statistically significant (P<0.05). The ALT and AST levels in the HIRI 0.5h, 3h, 6h, 12h, 24h, 48h group were higher than those of the sham operation group, and the differences were statistically significant (P<0.05). Pearson correlation analysis showed that AUC levels were negatively correlated with serum ALT and AST levels (r=-0.754, r=-0.808, P<0.001). There was a positive correlation between TP levels and serum ALT and AST levels (r=0.731, r=0.709, P<0.001). There was a positive correlation between MTT level and serum AST level (r=0.718, P<0.001). Conclusion: Contrast-enhanced ultrasound quantitative analysis of various parameters can accurately assess the changes of HIRI microcirculation in mice in real time to reflect its pathological changes, which is of great significance to the evaluation of HIRI. Among them, AUC is the best index for evaluating HIRI among the parameters of contrast-enhanced ultrasound. |
| Key words: Liver ischemia-reperfusion injury Contrast-enhanced ultrasound Quantitative analysis Dynamic evaluation |
