| 摘要: |
| 目的 制备载全氟戊烷/锰卟啉/紫杉醇的多功能纳米探针,评估其体外光声/超声/磁共振三模态成像及声动力治疗(SDT)联合化疗的效果。方法 采用双乳化法制备载全氟戊烷(PFP)/锰卟啉(MnTTP)/紫杉醇(PTX)的聚乳酸-羟基乙醇酸(PLGA)纳米粒(PFP-MnTTP-PTX@PLGA,FMP@P),检测其表面电位、平均粒径、药物包封率,并在透射电镜及扫描电镜下观察其形态。观察纳米粒的体外光声/超声/磁共振显像效果及活性氧产生能力。通过CCK-8法及流式细胞术验证FMP@P体外SDT联合化疗杀伤肿瘤细胞的效果。结果 FMP@P平均粒径为(323.1 ± 68.3)nm,表面电位为(-14.5 ± 8.3)mV,MnTTP及PTX包封率分别为93.55 ± 0.46%、81.23 ± 6.93%。纳米粒可以增强超声/光声/磁共振成像。经超声辐照后,4T1细胞内产生大量活性氧,可显著杀伤肿瘤细胞。结论 本研究成功制备了载全氟戊烷/锰卟啉/紫杉醇的多功能纳米粒,可体外增强光声/超声/磁共振显像,并介导SDT联合化学治疗。 |
| 关键词: 光声成像 超声成像 磁共振成像 声动力治疗 化疗 |
| DOI： |
| 投稿时间：2021-06-10修订日期：2021-08-18 |
| 基金项目:国家自然科学基金面上项目(82071926),国家自然科学基金重点项目(81630047),重庆市人民医院医学科技创新项目(2019ZDXM08) |
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| In Vitro Experimental Study of Drug-loaded Nanoparticles with Phase-changed Capability for Multimodal Imaging and Sonodynamic Therapy combined with Chemotherapy |
| ZHANG Yi,LUO Yuanli,LIU Dang,CAO Yang,RAN Haitao,YANG CHao |
| (Department of Ultrasound,The First Affiliated Hospital of Chongqing Medical University) |
| Abstract: |
| Objective To prepare poly (lactic-co-glycolic acid) (PLGA) based multifunctional nanoparticles loading with perfluoropentane (PFP), Mn (5,10,15,20-tetrakis (4-chlorophenyl) porphyrin) Cl (MnTTP) and paclitaxel (PTX), and to investigate their ultrasound (US)/photoacoustic (PA)/magnetic resonance (MR) imaging enhancement capability and chemo-/sonodynamic therapy (SDT) efficacy in vitro. Methods PFP/MnTTP/PTX loaded in PLGA nanoparticles (termed as FMP@P) were prepared by a double emulsification method. The physical properties of FMP@P including average diameter, surface zeta potential, morphological character, drug loading efficacy and in vitro reactive oxygen species (ROS) generation were determined. In addition, the in vitro PA/US/MR imaging performance of FMP@P was observed and recorded. Upon US irradiation, intercellular ROS generation was investigated using confocal microscopy and flowcytometry. Meanwhile, the chemo-/sonodynamic therapy effect on 4T1 cell was evaluated by the standard CCK-8 assay and flow cytometry. Results The average diameter and zeta potential of FMP@P were (323.1 ± 68.3) nm and -(14.5 ± 8.3) mV. The loading efficiency of MnTTP and PTX was 93.55 ± 0.46% and 81.23 ± 6.93%, respectively. FMP@P enhanced US/PA/MR imaging. Upon US irradiation, FMP@P could produce a large amount of reactive oxygen species (ROS), which induced obvious cytotoxicity. Conclusion A multifunctional FMP@P were successfully fabricated, which enhanced US/PA/ MR imaging, mediated chemo-/SDT. |
| Key words: photoacoustic imaging ultrasonic imaging magnetic resonance imaging chemotherapy sonodynamic therapy |
