【Abstract】Objective: To explore the differential value of ultrasonic radiography (CEUS) perfusion mode, ultrasonic elasticography (UE) and multi-layer spiral CT (MSCT) for benign and malignant thyroid nodules. Methods:Eighty-five (94 nodules) with thyroid nodules admitted from December 2018 to December 2020, and all patients were examined by CEUS, UE and MSCT. The value of CEUS, UE and MSCT in the diagnosis of benign and malignant thyroid nodules was analyzed. Results: In this study, there were 94 nodules in 85 patients with thyroid nodules, including 70 benign nodules and 24 malignant nodules diagnosed by pathology. CEUS examination showed that the benign thyroid nodules had clear boundary, regular shape, no small calcification, poor blood flow, circumferental ring, and hyperenhanced nodules were significantly higher than those in the malignant group (P<0.05); MSCT examination showed that the benign thyroid nodules were cystic, regular in shape, and no small calcification or nodules. The number of 2cm, uniform enhancement and clear edge nodule was significantly higher than that of malignant group (P<0.05); UE examination showed that the elastic score of benign thyroid nodules was mostly 0~2 points, while that of malignant nodules was mostly 3~4 points, and the difference of elastic score was statistically significant (Z=-6.360，P<0.05). In addition, the sensitivity and specificity of CEUS were 85.71% and 83.33%, the UE was 75.71% and 75.00%, the MSCT was 80.00% and 79.17%, respectively. The results of combined detection were 95.71% and 91.67%, both higher than those of single detection. Conclusion:Both CEUS, UE and MSCT have certain diagnostic value for benign and malignant thyroid nodules, but the joint detection value is higher.