Objective Female anal sphincter was developed and evaluated by high-frequency ultrasound to evaluate the feasibility of high-frequency ultrasound in the integrity evaluation of female anal sphincter. Methods Using high-frequency ultrasound to image the anal sphincter of 110 female subjects who underwent pelvic floor ultrasound from January 2020 to March 2021, the subjects’ anal sphincter were scanned continuously from the cross section and the sagittal plane. Two researchers with different experience Independently evaluate the integrity of the anal sphincter image in the case of mutual blindness, record the damage of the anal sphincter and perform statistical analysis. Results 80 subjects were able to perform imaging of the internal and external anal sphincter through the high-frequency linear array probe, which clearly showed the characteristic anatomical structure of each level of the anal canal from the cross-sectional and sagittal planes. Therefore, when abnormal ultrasound performance occurs, the anal sphincter injury can be detected. Through the Kappa consistency test, the evaluation of the integrity of the anal sphincter by high-frequency ultrasound has strong consistency among researchers with different experiences (Kappa=0.888, P<0.05).Conclusion High-frequency ultrasound can realize the evaluation of the integrity of the anal sphincter through clear imaging of each level of the anal canal, and provide a new imaging method for the evaluation of the anal sphincter.