| 摘要: |
| 目的 探讨脂质纳米氟碳液滴增效HIFU消融兔肝脏的空化活动及术后病理学变化。方法 首先制备全氟戊烷脂质纳米氟碳液滴(L-PFP)；然后将24只正常新西兰兔随机分为对照组(单纯HIFU组)和L-PFP组；在 B 超引导下进行HIFU定点消融兔肝脏(超声能量：900 J)；通过被动空化检测系统(PCD)监控空化活动；分别将消融即刻、1d、3d、7d的兔肝脏标本取出进行H E染色,观察消融灶转归过程中的病理学变化。结果 经耳缘静脉注射L-PFP后HIFU 辐照兔肝脏所产生的空化泡群更明显,其灰度变化值为对照组的1.93倍,累积瞬态空化剂量为对照组的6.3倍,空化活动表现强烈；大体病理及H E结果显示L-PFP组造成的组织损伤严重,细胞变性更为彻底,炎性反应更为强烈；单纯HIFU组消融灶7d修复为正常组织,转归所需时间显著短于L-PFP组。结论 脂质纳米氟碳液滴通过增强空化效应有效提高HIFU消融效果,延长消融灶转归所需时间。 |
| 关键词: 高强度聚焦超声 空化效应 被动空化检测 氟碳液滴 增效 |
| DOI： |
| 投稿时间：2021-11-27修订日期：2022-01-01 |
| 基金项目:国家自然科学基金青年科学基金(批准号：12004059) |
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| Experimental study on the ablation of rabbit liver by HIFU combined with lipid nano-fluorocarbon droplets |
| suntingyu,wangqi,yehemin,zengchao,lijingnan,lifaqi |
| () |
| Abstract: |
| Objective investigate the cavitation activity and postoperative pathological changes of rabbit liver after lipid nano-fluorocarbon droplets enhanced HIFU ablation. Methods Perfluoropentane lipid nanofluorocarbon droplets (L-PFP) were prepared. Then 24 normal New Zealand rabbits were randomly divided into control group (HIFU group) and L-PFP group. The rabbit liver was ablated by HIFU under the guidance of B-ultrasound (Ultrasonic energy: 900 J). The passive cavitation detection system (PCD) is used to monitor cavitation activities. H&E staining was performed on rabbit liver samples immediately after ablation, 1d, 3d and 7d, respectively, to observe the pathological changes in the process of ablation. Results After the injection of L-PFP through auricular vein, the cavitation bubbles in HIFU irradiated rabbit liver were more obvious. The gray level change value was 1.93 times that of the control group, and the cumulative transient cavitation dose was 6.3 times that of the control group. Gross pathology and H&E results showed that l-PFP group caused severe tissue damage, more complete cell degeneration and more intense inflammatory response. In the HIFU group, 7d ablation was used to repair normal tissues, and the time required for recovery was significantly shorter than that in the L-PFP group. Conclusion Lipid nano-fluorocarbon droplets can effectively improve the ablation effect of HIFU by enhancing the cavitation effect and prolonging the time required for the ablation. |
| Key words: High-intensity focused ultrasound Cavitation effect Passive cavitation detection Fluorocarbon droplets Synergy |
