Objective: The quantitative mitral valve technique of three-dimensional transesophageal echocardiography was applied to analyze the range of structural parameters of the mitral valve with normal intracardiac structure and function, and summarize their variation patterns with cardiac cycle in order to provide a theoretical basis for the assessment of mitral valve lesions. Methods: 2D images and real-time 3D full-volume images of the mitral valve were collected from 37 subjects with normal intracardiac structure and function, and the images were complete including mitral and aortic valve structures. The data include 18 parameters such as the annular diameter, annular height etc. All the data were recorded in 6 time phases of early, mid, and late diastole and early, mid, and late systole. analyze these data and determine their value reference ranges and analyze their motion regularity. Results: The reference range of mitral valve structures in the 37 subjects was summarized and found to vary regularly with cardiac cycle: some parameters have their maximum values occurring in diastole and their minimum values occurring in systole, such as anterior-posterior and left-right annular diameters, annular area, annular circumference, anterior and posterior annulus length etc. Another parameters have their maximum values occurring in systole and their minimum values occurring in diastole, such as annular height, nonplanar angle, AO-MV angle, and AO-MV midpoint distance. Unlike the previous structures, the maximum and minimum values of Tenting height and Tenting area for the 3 subdivisions of the valve leaflets appeared in early and late systole, respectively. Conclusion: The automated quantitative analysis system of three-dimensional transesophageal echocardiography can reconstruct the mitral valve model relatively quickly and intuitively, and automatically measure several structural parameters. In this study, the time phases of the maximum and minimum values of each parameter are analyzed and helped to deepen the understanding of the mitral valve structure and its motion pattern. The range of reference values summarized in this study provides a theoretical reference for determining mitral valve lesions. and lays the foundation for further research on the structural changes of mitral valve lesions.