Objective To discuss the value of Two-dimensional Speckle Tracking Echocardiography (2D-STE) in evaluating the change of right ventricular function in patients with coronary artery disease (CAD) complicated with right coronary artery stenosis. Methods Conventional echocardiography and Tissue Doppler ultrasonography were performed in 137 patients suspected with CAD. 2D-STE was used to measure the global longitudinal peak strain (GLS) of the right ventricle, the longitudinal peak systolic strain (ε), the peak systolic strain rate (SRs), the peak early diastolic strain rate (SRe) and peak late diastolic strain rate (SRa) of the free wall and ventricular septal side for basal, mid, and apical segment. According to the results of coronary angiography and whether there was right coronary artery stenosis, they were divided into control group (group A, coronary artery stenosis < 50%), CAD group without right coronary artery stenosis (group B) and CAD group with right coronary artery stenosis (group C). Results There were no significant differences in RVFAC, TAPSE, LVEF, SV, S’, Em and Am among the three groups (P > 0.05). There were significant differences in free wall basal, mid, apical ε, free wall GLS and SRs among the three groups (P < 0.05). There was no significant difference between group A and group B in the basal and mid segment ε of the ventricular septum (P > 0.05). Compared with group B, the basal and mid segment ε of ventricular septum was decreased in group C (P < 0.05). There were no significant differences in SRe, SRa and ventricular septal apical ε among the three groups (P > 0.05). Conclusions Right ventricular systolic function in CAD patients with right coronary artery stenosis has undergone subclinical changes. 2D-STE can effectively evaluate right ventricular dysfunction, providing new reference value for the early diagnosis and prognosis of right ventricular function in clinical CAD patients.