| 摘要: |
| 目的 探讨惰性气体微气泡联合低频低强度超声波连续辐照对人胰腺癌PANC-1细胞的抑癌作用。方法 取对数生长的浓度为1×106 /ml的人胰腺癌PANC-1单细胞悬液分成4组，具体为：空白对照组加入2 ml人胰腺癌PANC-1单细胞悬液；惰性气体微气泡组加入1600 μl人胰腺癌PANC-1单细胞悬液和400 μl惰性气体微气泡并充分混匀，使惰性气体微气泡浓度为20%；低频低强度超声波组加入2 ml人胰腺癌PANC-1单细胞悬液，再用声强0.60 W/cm2、频率1 MHz的低频低强度超声连续波连续辐照60 s；微气泡+超声波组加入1600 μl人胰腺癌PANC-1单细胞悬液和400 μl的惰性气体微气泡并充分混匀，使惰性气体微气泡浓度为20%，再以声强0.60 W/cm2、频率1 MHz的低频低强度超声连续波连续辐照60 s。采用细胞计数试剂法检测各组细胞增殖活性，应用分析型流式细胞仪测定各组细胞的凋亡情况。结果 空白对照组、惰性气体微气泡组、低频低强度超声波组、微气泡+超声波组人胰腺癌PANC-1细胞增殖活性分别为 0.9356±0.1652、1.1126±0.0738、0.3236±0.0126、0.1566±0.0137，微气泡+超声波组细胞增殖活性均低于其余各组，差异均有统计学意义（均P＜0.05）。空白对照组、惰性气体微气泡组、低频低强度超声波组、微气泡+超声波组细胞凋亡率分别为0.069±0.007、0.033±0.006、0.279±0.016、0.678±0.018，微气泡+超声波组细胞凋亡率均高于其余各组，差异均有统计学意义（均P＜0.05）。结论 惰性气体微气泡联合低频低强度超声波连续辐照能降低体外培养的人胰腺癌PANC-1细胞增殖，诱导癌细胞凋亡，具有明显的抑癌作用，为肿瘤治疗提供新的研究思路。 |
| 关键词: 超声辐照，低频，低强度 惰性气体微气泡 胰腺细胞 抑癌作用 |
| DOI： |
| 投稿时间：2022-04-21修订日期：2022-05-29 |
| 基金项目:市科技局科技计划（2018C30132） |
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| Experimental study on the anti-cancer effects of inert gas microbubbles combined with low frequency and low intensity ultrasound on human pancreatic cancer PANC-1 cells |
| Wang Liying,Jiang Tianan‘’ |
| () |
| Abstract: |
| Objective To investigate the anti-canter effects of inert gas microbubbles combined with low frequency and low intensity ultrasound irradiation on human pancreatic cancer PANC-1 cells. Methods The human pancreatic cancer PANC-1 single cell suspension with concentration 1 × 106 /ml were divided into 4 groups. The the blank control group was added with 2 ml human pancreatic cancer PANC-1 single cell suspension. The inert gas microbubble group was added 1600 μL human pancreatic cancer PANC-1 single cell suspension and 400 μL inert gas microbubbles and fully mixed to make the concentration of inert gas microbubbles 20%. In the low frequency and low intensity ultrasound group, 2 ml of human pancreatic cancer PANC-1 single cell suspension was added, and then irradiated with low frequency and low intensity ultrasound continuous wave with sound intensity of 0.60 w/cm2 and frequency of 1 MHz for 60 s. The microbubbles + ultrasound group was added 1600 μL human pancreatic cancer PANC-1 single cell suspension and 400 μL of inert gas microbubbles and fully mixed so that the concentration of inert gas microbubbles was 20%, and then irradiated continuously with low frequency and low intensity ultrasonic continuous wave with sound intensity of 0.60 w/cm2 and frequency of 1 MHz for 60 s. The proliferation activity of cells in each group was detected by cell counting reagent method, and the apoptosis of cells in each group was measured by analytical flow cytometry. Results The proliferative activity of human pancreatic cancer PANC-1 cells in the blank control group,the inert gas microbubble group, the low frequency low intensity ultrasound group and thw microbubble + ultrasound group were 0.9356 ± 0.1652, 1.1126 ± 0.0738, 0.3236 ± 0.0126 and 0.1566 ± 0.0137 respectively. The proliferative activity of the microbubble + ultrasound group was lower than that of the other groups, and the difference was statistically significant (all P < 0.05). The apoptosis rates of the blank control group, the inert gas microbubble group, the low-frequency low-intensity ultrasound group and the microbubble + ultrasound group were 0.069 ± 0.007, 0.033 ± 0.006, 0.279 ± 0.016 and 0.678 ± 0.018 respectively. The apoptosis rate of microbubble + ultrasound group was higher than that of the other groups, and the difference was statistically significant (all P < 0.05).Conclusion The inert gas microbubbles combined with low frequency and low intensity ultrasound irradiation can reduce the proliferation of human pancreatic cancer PANC-1 cells cultured in vitro, induce apoptosis of cancer cells, and have obvious anti-canter effects, providing a new research idea for tumor therapy. |
| Key words: Ultrasonic irradiation,low frequency,low intensity Inert gas microbubbles Pancreatic cells Carcinostasis |
