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| 不同占空比诊断超声联合微泡对乏血供肿瘤血流灌注的影响 |
| 姚雷,杨国良,殷佳蓓,张毅,罗婷婷,唐家伟,唐君辉,刘政 |
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| (陆军军医大学第二附属医院超声科) |
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| 摘要: |
| 目的 探讨不同占空比诊断超声联合微泡对大鼠乏血供肿瘤血流灌注的影响。 方法 选用wistar大鼠45只,于右侧背部皮下建立大鼠C6胶质细胞瘤模型,根据不同脉冲时间及间隔时间随机分为5组,每组9只：A组,脉冲时间5s,间隔时间1s；B组,脉冲时间1s,间隔时间1s；C组,脉冲时间1s,间隔时间5s；D组,脉冲时间5s,间隔时间5s；E组,超声假照。各组对应的治疗脉冲占空比分别为0.167%、0.1%、0.033%、0.1%和0。每组荷瘤大鼠经超声联合微泡治疗/超声假照10min,于治疗/假照前、治疗/假照后即刻分别行超声造影检查,获得时间-强度曲线(TIC),比较各组峰值强度(PI)、曲线下面积(AUC)；对比分析各组肿瘤超声造影图像进行超声造影视觉评分；各组随机选取3只大鼠取肿瘤组织行HE染色,观察治疗/假照后病理学变化。 结果 与组内治疗前比较,A组、B组治疗后AUC值增加,差异有统计学意义(t=-2.726,-3.922；P=0.026,0.004),各组治疗/假照后PI值及C组、D组、E组AUC值与组内治疗/假照前比较差异无统计学意义(均P>0.05)。与C组、D组、E组比较,A组、B组超声造影视觉评分值较高,差异有统计学意义(均P<0.05),余组间无明显统计学差异(均P>0.05)。A组、B组可见较明显的血管扩张,A组部分血管周围组织内可见少量红细胞渗出,C组、D组、E组血管扩张不明显。 结论 脉冲时间5s,间隔时间1s,占空比0.167%及脉冲时间1s,间隔时间1s,占空比0.1%的低强度诊断超声联合微泡可增强乏血供肿瘤血流灌注,后者为相对安全的超声治疗声学参数。 |
| 关键词: 超声处理 微气泡 空化效应 占空比 肿瘤 血流灌注 |
| DOI： |
| 投稿时间：2022-05-16修订日期：2022-05-31 |
| 基金项目:国家自然科学基金国家重大科研仪器研制项目(82127804) |
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| Effect of diagnostic ultrasound with different duty cycle combined with microbubbles on blood perfusion of tumor with lack of blood supply |
| YAO Lei,YANG Guoliang,YIN Jiabei,ZHANG Yi,LUO Tingting,TANG Jiawei,TANG Junhui,LIU Zheng |
| (Department of Ultrasound,the Second Affiliated Hospital of Army Medical University) |
| Abstract: |
| Objective To investigate the effect of diagnostic ultrasound with different duty cycle combined with microbubbles on blood perfusion of tumor with lack of blood supply in rats. Methods Forty five wistar rats were selected to establish a rat C6 glioma model subcutaneously on the right back. According to different pulse time and interval time, they were randomly divided into 5 groups with 9 rats in each group: group A, pulse time 5s, interval time 1s; group B, pulse time 1s, interval time 1s; group C, pulse time 1s, interval time 5s; group D, pulse time 5s, interval time 5s; group E, sham explore with ultrasound. The corresponding treatment pulse duty cycles of each group were 0.167%, 0.1%, 0.033%, 0.1% and 0, respectively. Each group of tumor bearing rats were treated with ultrasound combined with microbubbles/sham explore with ultrasound for 10 minutes. Contrast-enhanced ultrasound was performed before and immediately after treatment to obtain the time intensity curve (TIC), the peak intensity (PI) and area under the curve (AUC) of each group were compared; The contrast-enhanced ultrasound images of tumors in each group were compared and analyzed, and the contrast-enhanced ultrasound visual score was performed; Three rats in each group were randomly selected, and the tumor tissues were stained with HE to observe the pathological changes after ultrasonic treatment/sham ultrasonic explore. Results Compared with before treatment, the AUC value of group A and group B increased after treatment, and the difference was statistically significant (t=-2.726, -3.922; P= 0.026, 0.004). There were no significant differences in PI value and AUC value of group C, group D and group E after treatment (all P > 0.05). Compared with group C, group D and group E, the visual score of contrast-enhanced ultrasound in group A and group B was higher, the difference was statistically significant (all P<0.05), and there were no significant differences among the other groups (all P>0.05). Obvious vasodilation was observed in group A and group B, a small amount of red blood cell exudation was observed in some perivascular tissues in group A, and there was no obvious vasodilation in group C, group D and group E. Conclusion Low intensity diagnostic ultrasound combined with microbubbles with pulse time of 5s, interval time of 1s, duty cycle of 0.167% and pulse time of 1s, interval time of 1s and duty cycle of 0.1% can enhance the blood perfusion of tumor with lack of blood supply. The latter is a relatively safe acoustic parameter for ultrasonic treatment. |
| Key words: Sonication Microbubbles Cavitation Duty cycle Neoplasms Blood perfusion |
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