Objective：To explore the value of Two-dimensional speckle-tracking in evaluating right ventricular function in patients with varying degrees of tricuspid regurgitation. Methods: From March 2021 to December 2021, 78 patients and 39 healthy volunteers were diagnosed with TR in the Ultrasound Department of Yijishan Hospital, a total of 117 patients, and the patients were divided into 39 cases in the non-significant TR group and 39 cases in the significant TR group. Common echocardiographic parameters were measured by transthoracic echocardiography，For example：Tei index, TAPSE, RVFAC, IVA, S', right ventricular basal base segment inner diameter, right ventricular middle segment inner diameter, right ventricular long axis inner diameter, tricuspid valve ring end-diastolic diameter, TAC，and the RVGLS was obtain by 2D-STI , comparing the differences in the above sets of parameters.Results: Compared with the RVGLS values in the three groups, the significant TR group was the largest, the non-significant TR group was the second, and the normal control group was the smallest, and the difference was statistically significant (P<0.05); the difference between the Tei index, TAPSE, RVFAC and the significant TR group was statistically significant compared with the non-significant TR group and the normal control group (P<0.05), and the diameter of the right ventricular basal segment, the middle segment and the terminal diastolic diameter of the tricuspid valve ring were reduced compared with the non-significant TR group and the normal control group. The difference was statistically significant (P<0.05); compared with the three groups of TAC, the significant TR group was the smallest, the non-significant TR group was the largest, and the difference was statistically significant (P<0.05); RVGLS was positively correlated with the reflux neck and reflux area (r=0.643, 0.723), and RVGLS was negatively correlated with TAC (r = -0.573), and the differences were statistically significant (P<0.05). The critical value of RVGLS >-20% was determined by ROC curve as the reflux area ≥ 6.365cm2, the vena contracta width ≥ 3.15mm, the sensitivity was 94.3%, 91.4%, and the specificity was 86%、 86%, respectively.Conclusion: The right ventricular function in the non-significant TR group has been impaired, and the right ventricular function impairment in the significant TR group has been further aggravated. As a new technology, 2D-STI can detect changes in right ventricular function early, and RVGLS is significantly related to the degree of reflux, which expect become a reference index for early clinical diagnosis and treatment.