| 摘要: |
| 目的 探究超声联合缓释型载药PLGA 纳米粒对减毒牛结核分枝杆菌体内外增效杀菌作用。方法 采用双乳化法制备载左氧氟沙星PLGA缓释型纳米粒(LEV-NPs),通过平板菌落计数和扫描电镜评价超声联合LEV-NPs作用后细菌活性及形态结构变化,连续监测皮下结核肉芽肿体积变化评价体内治疗效果。结果 LEV-NPs呈球形,大小均一,具有药物缓释性能。超声联合LEV-NPs治疗后细菌活性显著降低,菌体结构破坏,皮下肉芽肿体积显著减小,差异具有统计学意义( P <0.05)。结论 超声介导LEV-NPs作用可显著提高LEV对减毒牛结核分枝杆菌的杀菌效果,二者具有协同增效作用。 |
| 关键词: 超声 PLGA纳米粒 左氧氟沙星 增效抗菌 |
| DOI： |
| 投稿时间：2022-06-28修订日期：2022-08-19 |
| 基金项目:重庆市研究生科研创新项目(编号：CYS21240)；贵州省卫生健康委科学技术(No.gzwjkj2020-1-042) |
|
| Ultrasound-mediated slow-release drug-loaded nanoparticles promote synergistic bactericidal effect of anti-tuberculosis drugs |
| Zhang Zhifei,Hu Can,Du Yonghong,Yang Min |
| () |
| Abstract: |
| Objective To investigate the synergistic and bactericidal effects of ultrasound combined with slow-release PLGA nanoparticles (LEV-NPS) on Bacille Calmette-Guerin (BCG) of attenuated bovine tuberculosis in vitro and in vivo. Methods Levofloxacin PLGA sustained release nanoparticles were prepared by double emulsification method. Bacterial activity and morphological and structural changes were evaluated by plate colony counting and scanning electron microscopy (SEM). Subcutaneous tuberculosis granuloma infection model was established, and the therapeutic effect was evaluated by continuous monitoring of granuloma volume change. Results LEV-NPs was spherical, uniform in size and had obvious sustained release properties. Compared with the control group and LEV alone group, ultrasound combined with LEV-NPS significantly reduced bacterial activity, destroyed bacterial structure, and reduced subcutaneous tuberculous granuloma volume, with statistically significant differences (P<0.05). Conclusions Ultrasound mediated slow-release PLGA nanoparticles (LEV-NPS) can significantly improve the bactericidal effect of LEV against BCG of attenuated bovine tuberculosis, which have significant synergistic effect. |
| Key words: Mycobacterium tuberculosis Ultrasound PLGA nanoparticles Levofloxacin Synergistic antimicrobial |
