| 摘要: |
| 摘 要 目的 初步探讨超声造影（CEUS）评价铁皮石斛对肝纤维化模型大鼠肾损伤时微循环灌注的应用价值。方法 SD 雄性大鼠 24 只随机分成正常组（NG）、模型组（MG）和铁皮石斛干预组（DG）每组8 只。除NG 以外，其余两组经CCl4 诱导制备肝纤维化动物模型。DG以高剂量铁皮石斛鲜榨汁（7.5 g /kg）灌胃，连续灌胃4 w。通过常规二维超声和超声造影（CEUS）观察肾的形态、大小变化和肾皮质血流灌注情况，定量分析感兴趣区时间-强度曲线（TIC）下的达峰时间（TTP）、峰值强度（PSI）、曲线上升斜率（A）和曲线下面积（AUC）。收集处死前一天24 h 尿液和处死后腹主动脉血液，应用全自动生化检测仪测定血肌酐（Cr）和血尿素氮（BUN），并计算内生肌酐清除率（Ccr），取肝、肾组织进行HE、Masson 染色观察病理学变化。结果 与NG 相比，MG 和DG 大鼠肝组织病理学检测显示肝纤维化明显，CEUS显示肾皮质TIC曲线的TTP 延长，PSI 和A 降低，AUC 增加（P＜0.05），Cr 和BUN 水平升高，Ccr下降（P＜0.05），提示肾功能损伤；与MG 比较，DG 大鼠肝组织病理学改变得以改善，TTP 缩短，PSI 和A 增高，AUC减少（P＜0.05），Cr 和BUN 水平下降，Ccr水平上升（P＜0.05）。常规二维超声和病理学检查显示，各组大鼠均未见肾的形态及组织病理明显改变。结论 高剂量铁皮石斛鲜榨汁能改善肝纤维化大鼠肾损伤的微循环灌注，并可通过超声造影TIC 参数予以实时定量评估。 |
| 关键词: 肝纤维化 铁皮石斛 超声造影 肾脏血流灌注 时间-强度曲线 |
| DOI： |
| 投稿时间：2022-07-15修订日期：2022-08-19 |
| 基金项目:2022年云南省教育厅科学研究基金(编号：2022Y809)；2022年度云南省教育厅科学研究基金面上项目(NO：2022J0695)；2021年云南省地方本科高校基础研究联合专项资金面上项目(编号：202101BA070001-101)；2021年云南省教育厅科学研究基金(编号：2021J0382)；2021年大理市科技计划项目(NO：2021KBG009)。 |
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| Application value of contrast-enhanced ultrasound in evaluating the effect of Dendrobium candidum on renal injury in rats with hepatic fibrosis |
| LI Hongjun,WANG Jing,FAN Qin,CHENG Jiamao,LIU Qingqing,CHEN Haiyan,HONG Wenjuan,YU Zhuorui |
| (The First Affiliated Hospital of Dali University) |
| Abstract: |
| ABSTRACT Objective To explore the value of contrast-enhanced ultrasound in the evaluating microcirculation perfusion of Dendrobium candidum in the hepatic fibrosis model rats with renal injury. Methods Twenty four male SD rats were randomly divided into normal group (NG), model group (MG) and Dendrobium officinale group (DG), with 8 rats in each group. Except NG, The other two groups of rats were made into CCl4-induced hepatic fibrosis models. DG rats are administered with the high-dose fresh expressed juice of Dendrobium candidum (7.5 g/kg ) for 4 weeks. The shape and size changes of the kidney and the blood perfusion of the renal cortex are observed separately by conventional two-dimensional ultrasound and contrast-enhanced ultrasound (CEUS), and the time to peak (TTP), peak intensity (PSI), ascending slope (A) and area under curve (AUC) under the time intensity curve (TIC) of the region of interest are quantitatively analyzed. 24 hours urines before execution and abdominal aorta bleed after execution are collected for testing the blood creatinine (Cr) and blood urea nitrogen (BUN) by automatic biochemical detector, and calculating the endogenous creatinine clearance rate (Ccr). Liver and kidney tissues are taken for HE and Masson staining to observe the pathological changes. Results Compared with NG, the histopathological examination of the livers in MG and DG rats showed the liver fibrosis was obvious; CEUS examination showed that TTP prolonges, PSI and A are decrease, AUC increases in the renal cortical TIC curve (P<0.05), and Cr and BUN levels increase, Ccr decreases (P<0.05), which suggest that kidney function is impaired; Compared with MG, the pathological changes of liver tissues in DG rats are improved, TTP shorten, PSI and A increase, AUC decreases (P<0.05), Cr and BUN levels decrease, and Ccr levels increases (P<0.05). Routine two-dimensional ultrasound and pathological examination showed that there have no obvious changes in the morphology and histopathology of the rat kidneys in all groups. Conclusion High-dose fresh extract of Dendrobium candidum can improve the microcirculation perfusion of renal injury in rats with hepatic fibrosis, which can be evaluated in a real-time quantitative way by TIC parameters of CEUS. |
| Key words: Hepatic fibrosis Dendrobium candidum Contrast-enhanced ultrasound Renal perfusion Time-intensity curve |
