Objective To explore the evaluation value of three-dimensional ultrasound quantitative parameters combined with anti-Müllerian hormone (AMH) for ovarian function in patients after hysterectomy. Methods A total of 198 patients undergoing hysterectomy with reservation of bilateral accessory in the hospital were enrolled as study group between March 2018 and March 2021. Before surgery, at 1 month, 3 months and 12 months after surgery, all underwent examination of vaginal three-dimensional ultrasound to collect related quantitative parameters [ovarian volume (OV), peak systolic velocity (PSV) and vascularization index (VI) of ovarian stroma], sex hormones [follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2)] and AMH. The evaluation value of the above indexes for ovarian function was analyzed. A total of 100 healthy women undergoing gynecological examinations during the same period were enrolled as control group. Results Before surgery, there was no significant difference in OV, PSV or VI between study group and control group (P>0.05). At 1 month, 6 months and 12 months after surgery, OV, PSV and VI in study group were decreased (P<0.05). As time went by, OV was gradually decreased (P<0.05). PSV and VI at 12 months after surgery were lower than those at 1 month and 6 months before surgery (P<0.05). Before surgery, there was no significant difference in FSH, LH, E2 or AMH between study group and control group (P>0.05). At 1 month, 6 months and 12 months after surgery, FSH was increased, while AMH was decreased in study group (P<0.05). There was no significant difference in FSH at different time points after surgery in study group (P>0.05). As time went by, AMH was gradually decreased (P<0.05). E2 at 12 months after surgery was lower than that before surgery, at 1 month and 6 months after surgery (P<0.05). In the 198 patients during the 1 year of follow-up, there were 59 cases with obvious menopausal syndromes (hot flashes, irritability, insomnia, anxiety, lost in emotions), including 19 cases with syndromes at 6 months after surgery and 40 cases at 12 months after surgery. Conclusion Three-dimensional ultrasound can directly reflect morphology and blood supply of ovary. AMH level has good stability and sensitivity. Clinically, three-dimensional ultrasound combined with AMH can evaluate ovarian function of patients after hysterectomy to improve clinical evaluation value.