Purpose To investigate the predictive value of endometrium and endometrial-myometrial interface (EMI) ultrasound parameters for pregnancy outcomes of in vitro fertilization - embryo transfer (IVF-ET) patients. Methods A retrospective study was conducted on 112 infertile women who received IVF-ET in Reproductive Medicine Department of our hospital. The routine ultrasound examination and three-dimensional power doppler ultrasound (3D-PDUS) were used on those patients to monitor the endometrium and EMI morphology and blood flow on ET day. Subsequently, all of the patients were divided into two groups respectively correspond to pregnant group and non-pregnant group according to whether they were pregnant or not. Therefore, the differences of two groups can be compared with, and finally the predictive value of EMI ultrasound parameters for IVF-ET pregnancy outcome was evaluated. Results The average EMI thickness, EMI morphology, EMI volume, intima VI, intima FI and intima VFI of two groups had statistical significance (P < 0.05). The endometrial FI, average EMI thickness and EMI morphology were independent influencing factors of pregnancy outcome, hence, a prediction model can be built as follows: Logit (P) = 0.227 × endometrial FI + 1.608 × average EMI thickness - 1.056 × EMI morphology - 8.315. After evaluation, this model had a good predictive effect on pregnancy outcome. Conclusion Endometrium and EMI ultrasound parameters have predictive value for the pregnancy outcome of IVF-ET patients.