ABSTRACT Objective: To investigate the value of quantitative analysis of contrast-enhanced utrasound (CEUS) in the diagnosis of varicocele (VC). Methods: 27 patients with left-sided VC in the hospital were selected as the study group, and 27 healthy male volunteers were selected as the control group during the same period. All participants had CEUS performed on both spermatic veins, and quantitative parameters were obtained: transiting time from spermatic artery to spermatic vein (TT),arrival time in vein (ATV), time-taking to peak in vein (TTPV), peak time in vein (PTV),wash-out time 50% in vein (WOT50%), and half time in vein (HTV). Differences between the two groups were compared. Results: The differences in TT, ATV, TTPV, PTV, WOT50% and HTV between the left and right spermatic veins in the study group were statistically significant (all P<0.05); the differences in the quantitative parameters between the left and right spermatic veins in the control group were not statistically significant. The differences in TT, ATV, TTPV, PTV, WOT50% and HTV of the left spermatic vein between the study group and the control group were statistically significant (all P<0.05); the differences in the quantitative parameters of right spermatic vein between the two groups were not statistically significant. Conclusion: The quantitative parameters of CEUS can reflect the hemodynamic situation of the spermatic vein and have some application in the diagnosis of VC.