| 摘要: |
| 目的 探讨超声联合微泡是否协同卡铂诱导人肺癌A549细胞凋亡。方法 设置对照组、US组、USMB组、CBP组、CBP+US组、CBP+USMB组,其中卡铂采用最适宜剂量50μg/ml,超声辐照参数采用最适宜声强0.6W/cm2,辐照时间60s。处理后培养24小时,每组分别消化、离心、收集3个独立样本,加入FITC标记的Annexin-V室温避光30 min,再加入PI避光反应5 min,应用流式细胞仪检测各组A549细胞凋亡率。应用SPSS 26.0分析各组凋亡率。结果 A549细胞总凋亡率由高到低依次为CBP+USMB组、CBP+US组、CBP组、USMB组、US组。CBP+US组与CBP组比较,早期凋亡率差异无统计学意义(P=0.964)。CBP+USMB组与CBP组、CBP+US组比较,早期凋亡率差异均有统计学意义(均P<0.01)。CBP+US组、CBP+USMB组与CBP组两两比较,晚期、总凋亡率差异均有统计学意义(均P<0.01)。 结论 US、USMB联合卡铂化疗可以协同诱导A549细胞凋亡。 |
| 关键词: 超声 微泡 凋亡 空化效应 卡铂 |
| DOI： |
| 投稿时间：2022-12-14修订日期：2022-12-27 |
| 基金项目:国家自然科学基金(81371553) |
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| Experimental Study of Effects of Ultrasound Combined with Microbubbles on Apoptosis of A549 Cells Treated by Carboplatin Chemotherapy |
| LI Yue,JIANG Shuai,GONG Siming,YANG Xi,HE Ying,CAO Junying,ZHUO Zhongxiong |
| (General Hospital of Northern Theater Command) |
| Abstract: |
| ABSTRACT Objective To investigate whether ultrasound combined with microbubbles and carboplatin can induce apoptosis of human lung cancer A549 cell line. Methods The control group, US group, USMB group, CBP group, CBP + US group and CBP + USMB group were set, in which carboplatin was used at an optimal dose of 50 μg/ml, ultrasound irradiation was used at an optimal intensity of 0.6 W / cm2, and irradiation time was 60s. After 24 hours of treatment, the cells in each group were collected. FITC-labeled Annexin-V was added for 30 minutes, and then PI was added to protect from light for 5 minutes. Then, flow cytometry was applied. The apoptosis rate of A549 cells was detected. The SPSS 26.0 software was used to analyze the apoptosis rate of each group. Results The total apoptosis rate was ranked as CBP+USMB group>CBP+US group>CBP group>USMB group>US group. There was no significant difference in the early apoptosis rate between the CBP + US and CBP groups (P=0.964). The early apoptosis rates were significantly different between the CBP + USMB group and the CBP group or the CBP + US group (all P < 0.01). Pairwise comparison showed that there were significant differences in the late and total apoptosis rates among the CBP + US, CBP + USMB, and CBP groups (all P<0.01). Conclusion US or USMB combined with carboplatin chemotherapy can synergistically induce the apoptosis of A549 cells. |
| Key words: Ultrasound Microbubbles Apoptosis Ultrasonic cavitation Carboplatin |
