| 摘要: |
| 摘 要 目的 制备一种近红外荧光多功能纳米粒，评估其体外超声、光声成像能力以及协同高强度聚焦超声（HIFU）消融牛肝的效果，通过空化检测探讨其增效HIFU的机制。方法 采用超声薄膜法制备载 IR780和全氟己烷（PFH）的脂质体纳米粒(IR780-CLs)，使用透射电镜观察纳米粒的结构特征，多角度粒度分析及Zeta电位仪检测纳米粒的粒径和电位，紫外分光光度计检测IR780包封率，观察其体外超声、光声成像效果，通过离体牛肝实验评估IR780-CLs体外增效HIFU的作用，并通过被动空化检测，取宽带噪声信号幅度的均方根值（RMS）随时间的变化曲线反应实时空化信号。结果 制备的IR780-CLs纳米粒呈大小均匀的球形，平均电位(34.5 ± 3.2) mV，粒径(246.5 ± 12.4) nm，IR780包封率为88%，IR780-CLs纳米粒具有良好的体外超声、光声成像效果，纳米粒光声信号强度与浓度间存在良好的线性关系，体外牛肝实验表明其具有显著增效HIFU的作用，被动空化检测进一步验证了IR780-CLs组空化信号RMS值高于PBS组。结论 本实验成功制备多功能纳米粒IR780-CLs，具有良好的超声、光声成像能力，并能显著提高HIFU消融牛肝的效率，空化效应为增效HIFU的主要机制。 |
| 关键词: 纳米粒 高强度聚焦超声 超声成像 光声成像 空化效应 |
| DOI： |
| 投稿时间：2023-02-25修订日期：2023-03-26 |
| 基金项目: |
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| In vitro experimental study of multimodal imaging and enhanced high intensity focused ultrasound with IR780/PFH nanoparticles |
| duyan |
| () |
| Abstract: |
| ABSTRACT Objective To prepare a near infrared fluorescent multifunctional nano particle, and to evaluate its in vitro ultrasound, photoacoustic imaging ability and the effect of ablation of bovine liver with high intensity focused ultrasound (HIFU), and to explore the mechanism of its synergistic effect on HIFU through cavitation detection.Methods IR780 and PFH coloaded?lipid?nanoparticles (IR780-CLs)?were prepared using film-ultrasonic?method,the structural characteristics of nanoparticles were observed by transmission electron microscopy, the particle size and potential of nanoparticles were detected by multi angle particle size analysis and Zeta potentiometers, and encapsulation efficiency of IR780 was detected by ultraviolet spectrophotometer. The effect of ultrasound and photoacoustic imaging were observed in vitro,in vitro bovine liver experiment was used to evaluate the synergistic effect of IR780-CLs on HIFU in vitro, and through passive cavitation detection, the change curve of root mean square (RMS) of broadband noise signal amplitude with time was taken to reflect real-time cavitation signal.Results The synthesized IR780-CLs nanoparticles were spherical and uniform, with an average surface potential of (34.5 ± 3.2) mV and diameter of (246.5 ± 12.4) nm, and encapsulation efficiency of IR780 was 88%. IR780 CLs nanoparticles had good ultrasound and photoacoustic imaging effects in vitro. There was a good linear relationship between photoacoustic signal intensity and concentration of nanoparticles. In vitro bovine liver experiment showed that it had a significant synergistic effect on HIFU. Passive cavitation detection further verified that the RMS value of cavitation signal in IR780-CLs group was higher than that in PBS group.Conclusion In this experiment, multifunctional nano particles IR780-CLs were successfully prepared, which have good ultrasound and photoacoustic imaging capabilities, and can significantly improve the efficiency of HIFU ablation of bovine liver. Cavitation effect is the main mechanism of enhancing the efficiency of HIFU. |
| Key words: Nanoparticles High-intensity focused ultrasound Ultrasound imaging Photoacoustic imaging Cavitation effect |
