| 摘要: |
| 目的 探讨剪切波弹性成像技术(SWE)在诊断大鼠非酒精性脂肪肝(NAFLD)的价值。方法 将52只雄性SD大鼠随机分为对照组12只和实验组40只,实验组分别喂养蛋氨酸和胆碱缺乏饲料1w、2w、3w和4w(各10只)构建不同程度的NAFLD模型,造模完成后麻醉及处死状态进行剪切波弹性成像(SWE)测量肝脏硬度(LSMa、LSMb),计算测量结果标准差(SD),解剖肝脏SWE检测离体肝脏硬度(LSMc),切除部分肝脏行病理学检查。应用受试者工作特征曲线(ROC)下面积(AUC)评估不同状态下SWE诊断NAFLD的效能。结果 病理检测大鼠正常组17只,脂肪变性组35只。正常组与脂肪变性组麻醉状态肝脏硬度检测结果标准差(SD)为[(1.70±0.56)kPa、(1.86±1.21)kPa],显著高于处死SD[(0.92±0.65) kPa、(1.08±0.73)kPa,P＜0.05],均显著高于离体SD[(0.26±0.16) kPa、(0.33±0.16)kPa,P＜0.05]；脂肪变性组LSMb为(3.95±1.78)kPa,显著高于正常组[(2.91±1.53)kPa,P＜0.05],脂肪变性组LSMc为(1.53±0.32)kPa,显著高于正常组[(1.23±0.16)kPa,P＜0.05],脂肪变性组LSMa与正常组无显著差异(P＞0.05)；LSMb、LSMc诊断肝脂肪变性AUC分别为0.69、0.86,敏感性特异性分别为60.0%、60.0%和82.4%、100%。结论 NAFLD大鼠LSM显著高于正常组,优化仪器性能及测量方法后,SWE在诊断大鼠NAFLD将具有良好的效能,值得进一步研究。 |
| 关键词: 剪切波弹性成像 非酒精性脂肪肝 诊断 离体测量 大鼠 |
| DOI： |
| 投稿时间：2023-02-27修订日期：2023-05-28 |
| 基金项目:南京军区医学科技创新课题(12MA030) |
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| Study on the value of shear wave elastography in the diagnosis of nonalcoholic fatty liver disease in ratsShi Ying1,2 Yang Zengdi2 Lyu Yongyan2 Xu Cuiping2 Tong Qingping1,2 |
| shiying,tonqingping |
| (联勤保障部队第901医院) |
| Abstract: |
| Objective The aim of this study was to explore the value of shear wave elastography in the diagnosis of nonalcoholic fatty liver in rats. Methods 52 male SD rats were randomly divided into control group (n=12) and experimental group (n=40). The experimental group was fed with methionine and choline deficiency diet for 1 week, 2 weeks, 3 weeks and 4 weeks to establish NAFLD models. At end of each experiment, the liver stiffness measurement (LSMa、LSMb) was measured by shear wave elastography under anesthesia and after execution. The standard deviation (SD) of the measurement results was calculated. The SWE of the liver was dissected to detect the liver stiffness measurement (LSMc)in vitro. Part of the liver was resected for pathological examination. The area under the receiver operating characteristic curve was used to evaluate the efficacy of SWE in diagnosing NAFLD under different conditions. Results Pathological examination showed that there were 17 rats in normal group and 35 rats in steatosis group. The standard deviation of liver hardness during anesthesia in normal group and steatosis group was[(1.70±0.56)kPa、(1.86±1.21)kPa], much higher than that SD in execution[(0.92±0.65) kPa、(1.08±0.73)kPa,P＜0.05],all much higher than that SD in vitro measurement [(0.26 ±0.16) kPa, (0.33 ±0.16) kPa, P<0.05].The LSMb and LSMc of steatosis group was[(3.95±1.78)kPa、(1.53±0.32)kPa],much higher than the LSMb and LSMc in normal group [(2.91±1.53)kPa、(1.23±0.16)kPa,P＜0.05].There was no significant difference in LSMa between steatosis group and normal group(P＞0.05).The areas under the ROC curve (AUC) by LSMb and LSMc in predicting Steatosis of liver were0.69 and 0.86, with the sensitivities and specificities of 60.0%、60.0% and 82.4%、100.0%. Conclusions The LSM of NAFLD rats is significantly higher than that of the normal group. After optimizing the instrument performance and measurement methods, SWE will have a good efficacy in the diagnosis of non-alcoholic fatty liver disease in rats. |
| Key words: Shear wave elastography No-alcoholic fatty liver disease Diagnosis In vitro measurement Rats |
