| 摘要: |
| 目的 探讨脊髓横断损伤(SCI)后神经源性膀胱(NB)模型大鼠的超声影像及超微结构的变化特点。方法 将27只SPF级SD大鼠按照随机数字表分为空白组(8只)和实验组(19只),实验组分为假手术组(8只)和模型组,模型组制备脊髓横断损伤后神经源性膀胱模型,剔除制备模型失败的3只,模型组剩余8只。在术后第28天对各组大鼠进行排尿后麻醉及清醒状态下超声观察膀胱径线与膀胱壁厚度；术后第四周检测各组大鼠尿动力学改变；术后第四周摘取膀胱逼尿肌组织进行染色观察。结果 ①与空白组相比,模型组尿量增多,下肢BBB评分降低,假手术组和空白组之间无明显差异；②HE染色见空白组膀胱组织基本正常,模型组膀胱病理改变明显,假手术组和空白组之间无明显差异；③超声诊断学表示：与空白组,模型组膀胱充盈度增大、膀胱壁厚度增加,假手术组和空白组之间无明显差异；②尿动力学结果表示：假手术组和空白组之间无明显差异,与空白组相比,模型组大鼠漏尿点压、灌注时间、膀胱最大容量、膀胱最大压力均显著升高(P＜0.0001)；结论 本研究初步观察到SCI后NB模型大鼠膀胱组织在功能、超声影像结果及组织形态上变化具有一致性,可以初步得出结论,即超声影响学资料可以作为判断NB模型大鼠膀胱组织变化的可视化资料之一。 |
| 关键词: 神经源性膀胱 超声 形态结构 |
| DOI： |
| 投稿时间：2023-05-12修订日期：2023-08-15 |
| 基金项目:]国家自然科学基金面上项目(82274666)；国家自然科学基金面上项目(81874510)；湖南中医药大学学科建设揭榜挂帅项目(22JBZ013) |
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| Changes in ultrasound imaging and histomorphology in rats with neurogenic bladder model |
| ZHAN Lifen,ZHUO Yue,AI Kun,ZENG Xuejiu,LIANG Roujun,DING Qiangsheng,LIU Xi ✳,HU Xiaomei,ZHANG Hong |
| () |
| Abstract: |
| Objective To investigate the characteristics of ultrasound images and ultrastructural changes in neurogenic bladder (NB) model rats after spinal cord transection injury (SCI). Methods Twenty-seven SPF-grade SD rats were divided into blank group (8 rats) and experimental group (19 rats) according to random number table, and the experimental group was divided into sham-operated group (8 rats) and model group. The neurogenic bladder model after spinal cord transection injury was prepared in the model group, and the 3 rats that failed to prepare the model were excluded, leaving 8 rats in the model group. The bladder diameter and bladder wall thickness were observed by ultrasound under postoperative anesthesia and awake state on the 28th day after surgery; the urodynamic changes were detected in the fourth week after surgery; the bladder forceps muscle tissues were removed for staining on the fourth week after surgery. Results ① Compared with the blank group, the urine volume increased and the BBB score of the lower limbs decreased in the model group, and there was no significant difference between the sham-operated group and the blank group; ② HE staining showed that the bladder tissue was basically normal in the blank group, and the bladder pathological changes were obvious in the model group, and there was no significant difference between the sham-operated group and the blank group; ③ Ultrasonic diagnostics indicated that the bladder filling increased and the bladder wall thickness increased in the model group compared with the blank group, and there was no significant difference between the sham-operated group and the blank There was no significant difference between the groups; ④Urodynamic results indicated that there was no significant difference between the sham-operated and blank groups, and compared with the blank group, the leakage point pressure, perfusion time, maximum bladder volume, and maximum bladder pressure were significantly higher in the model group rats (P < 0.0001); Conclusion In this study, we initially observed that the changes in bladder tissue in the NB model rats after SCI in terms of function, ultrasound imaging findings, and histomorphology There is consistency, and it can be tentatively concluded that the ultrasound impact science data can be used as one of the visualization data to determine the changes of bladder tissue in the NB model rats. |
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