摘要: |
目的 通过对离体猪胃标本进行实验,验证胃壁超声、显微外科解剖和组织学分层的对应关系。
方法 (1)对比观察高频超声、外科显微镜以及组织切片下猪胃壁层次,并将发丝分层穿入,验证超声声像图胃壁的分层对应关系。(2)清除猪胃表层粘液,观察超声下第一层强回声变化。(3)使用小动物超声(超声生物学显微镜),于40MHZ探头下观察猪胃壁第三层强回声。
结果(1)猪胃壁的组织结构与人体胃壁相同。(2)超声声像图显示胃壁为强-低-强-低-强五层回声:第一层为粘液、表面上皮及其与前后界面反射层,第二层低回声为粘膜固有层,第三层强回声为黏膜肌、粘膜下层及其与前后界面反射层,第四层低回声为肌层,第五层为浆膜及其与前后界面反射层。(3)20~40MHZ超声探头观察猪胃壁,第三层强回声中可明显探及一低回声,证实为粘膜肌层,形成了强回声和低回声相间的七层回声。
结论 高频超声可较好地显示胃壁的组织层次,为诊断胃壁各层病变提供解剖学依据。 |
关键词: 超声 胃壁 组织学 分层 显微外科 |
DOI: |
投稿时间:2023-06-07修订日期:2023-07-01 |
基金项目: |
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Comparative Study of Ultrasound, Microsurgical Anatomy and Histology of Gastric Wall |
Xue Jingjing,Wang Zecheng,Chen Yongjian,Li Shaohui,Lyu Guorong |
(Department of Ultrasound Medicine,the Second Affiliated Hospital of Fujian Medical University) |
Abstract: |
Objective To verify the relationship between ultrasound, microsurgical anatomy and histological stratification of the gastric wall by experiments on isolated pig stomach specimens. Methods (1)The layers of gastric wall were observed under high-frequency ultrasound, surgical microscope and histological section, and the hair was inserted into the gastric wall in different layers to verify the corresponding relationship of the gastric wall with ultrasound image. (2) After cleaning the pig stomach surface mucosa , the first layer of strong echo was observed under ultrasound.(3)Using high resolution small animal ultrasonic imaging system(Ultrasound biology microscope),and observe the third layer strong echo of pig stomach wall ultrasound with 40MHZ ultra-high frequency probe. Results (1)The histological structure of pig stomach wall is the same as that of the human stomach. (2) Ultrasonography revealed five layers (strong-low-strong-low-strong echo) of the gastric wall: the first layer was mucus, the surface epithelium, and the reflecting layer of the anterior and posterior interface;the second layer was the lamina propria,;the third strong echo layer is muscularis mucosae, submucosa and its anterior-posterior interface;the fourth low echo layer is muscularis;the fifth is serosa and its anterior-posterior interface. (3)20~40MHZ ultrasound probe to observe the pig stomach wall, the third layer of strong echo can obviously detect a low echo, confirmed as mucosal muscular layer, forming a strong echo and low echo seven layers of echo.Conclusion High-frequency ultrasound can display the tissue layers of gastric wall and provide anatomic basis for the diagnosis of gastric wall lesions. |
Key words: Ultrasound, Gastric wall, Histology, Stratification, Microsurgery |