Abstract: [Objective] To evaluate the parameters of adenosine loading echocardiography (ASE) combined with myocardial work (MW) to evaluate the systolic function of myocardial segment in patients with different degrees of stenosis in in-stent restenosis after percutaneous coronary intervention (PCI).[Methods] In 41 patients with restenosis after PCI in our hospital, using coronary angiography results, the model of 18 segments excluding the adenosine load, 726 myocardial segments were divided into no significant stenosis (stenosis degree <50%), moderate stenosis (55% 75%) and severe stenosis (stenosis> 75%). The longitudinal strain (LS), myocardial useful work (CMMW), useless work (WW), MW index (MWI), and MW rate (MWE) of myocardial segments before and after adenosine load; draw the receiver operating characteristic (ROC) curve to analyze the parameters before and after adenosine load to evaluate the efficacy of severe coronary artery stenosis after PCI.[Results] Statistical differences between ultrasound strain and myocardial work parameters in different degrees of coronary stenosis (P <0.01), in the load state, except for WW, the more severe the coronary artery stenosis is, the smaller the MW parameter value of the corresponding myocardial segment is (P<0.01); LS, MWI, MWE, and CMW increased in non-severe coronary stenosis group compared with basal state, while WW decreased compared with basal state (P <0.05); LS, MWI, MWE, and CMW decreased in severe coronary stenosis, and WW increased compared to basal (P <0.05). The ROC area under the curve (AUC) of load MWI, MWE and CMW increased compared with the base state. The AUC of severe coronary artery stenosis after PCI was 0.841, which was higher than load MWI, MWE and WW (0.829,0.766 and 0.685, respectively), with a cut-off value <1583mmHg%, sensitivity was 79% and specificity was 75%.[Conclusion] Adenosine load echocardiography combined with myocardial work parameters can effectively evaluate the systolic function of myocardial segments in patients with different stenosis degrees in restenosis after PCI, which has good application value in clinical diagnosis and treatment evaluation.