Objectives: Exploring the application of real-time three-dimensional echocardiography (3DEcho) in the evaluation of postoperative remodeling of right ventricle in Ebstein anomaly (EA). Methods: From October 2015 to August 2022, clinical data, 3DEcho, and cardiac magnetic resonance (CMR) imaging results of 22 consecutive EA patients who underwent surgical treatment were retrospectively studied in Fuwai hospital. Results: The median age of 22 patients was 30.7(14.1, 38.4) years. The median follow-up time was 22.9(6.5, 35.6) months. From 3DEcho, the indexed RV volumes was closely correlated with CMR imaging (r=0.908, P=0.012), the baseline right ventricular ejection fraction (RVEF) (r=0.621, P=0.002) and postoperative RVEF (r=0.567, P=0.009) were both moderately positively correlated and statistically significant. Compared with baseline, the degree of tricuspid regurgitation decreased (P<0.01), and the indexed RV volumes decreased (98.4±54.8 vs. 188.5±114.5 ml/m2, P<0.01), with a significant difference. There was no significant change in RVEF (postoperative 41.2±7.9% vs. baseline 44.6±6.7%, P=0.215). Conclusions: 3DEcho can accurately evaluate the RV volume and function of EA patients and play an important role in postoperative follow-up evaluation of right ventricular remodeling.