Objective: To explore the significance of two-dimensional speckle-tracking echocardiography (2D-STE) in a rat myocardial infarction model and to study the reliability and clinical value of 2D-STE in detecting myocardial infarction in rats.Method: Thirty-six male SD rats, aged 6-8 weeks, were randomly divided into three groups: the myocardial ischemia-reperfusion injury group (I/R group) (n=15), the myocardial ischemia-reperfusion injury with ferroptosis inhibitor group (Fer-1+I/R group) (n=11), and the sham operation group (Sham group) (n=10).The differences in modified Simpson-related parameters and 2D-STE-related parameters were measured and compared among the groups 24 hours after surgery. The sensitivity of 2D-STE in assessing myocardial ischemia-reperfusion injury was validated using TTC staining results for myocardial infarction.Results: Among the modified Simpson-related parameters, only the change in LVEF was statistically significant (P＜0.05), while EDV, ESV, and SV showed no statistical significance (P＞0.05).The 2D-STE technique showed statistically significant results for left ventricular circumferential strain-related parameters (GS, ANT, ANT Sept, POST Sept) (P＜0.05).By applying the dimensionality reduction machine learning algorithm, Lasso regression, the final R2 score reveals that the 2D-STE parameters outperform the modified Simpson parameters, with ANT demonstrating the best performance.Conclusion: The 2D-STE technique is more effective than the modified Simpson method in evaluating the protective effects of Fer-1 on rat myocardial injury. ANT is a particularly sensitive monitoring indicator.