Objective To explore multi-modal ultrasound features of patients with protruding gallbladder lesions, and analyze the diagnostic value of multi-modal ultrasound for protruding gallbladder lesions. Methods: One hundred patients with protruding gallbladder lesions who were admitted to the hospital from January 2021 to January 2024 were selected as the research subjects. All of them underwent contrast-enhanced ultrasound (CEUS) and superb microvascular imaging (SMI) examinations. CEUS and SMI features and examination results were compared. The value of CEUS, SMI and their combination in diagnosing protruding gallbladder lesions was analyzed. Results Pathological results showed that there were 100 protruding gallbladder lesions in patients included in this study, including 59 non-tumor lesions and 41 tumor lesions. The consistency between CEUS examination, SMI examination and the gold standard was good (Kappa value=0.734, 0.655). There were statistically significant differences between non-tumor lesions and tumor lesions in terms of regression mode, enhancement level, vascular morphology, gallbladder wall integrity, and blood flow signal classification (P<0.05). CEUS detected 56 non-tumor lesions and 44 tumor lesions, while SMI detected 54 non-tumor lesions and 46 tumor lesions. The accuracy of CEUS, SMI, and their combination for diagnosing protruding gallbladder lesions showed statistically significant differences (P<0.05). However, there was no statistically significant difference in sensitivity, specificity, positive predictive value, or negative predictive value (P>0.05). The specificity, accuracy, and positive predictive value of joint diagnosis were higher than those of SMI, and the accuracy was higher than that of CEUS (P<0.05). Conclusion: Multi-modal ultrasound can improve the accuracy in diagnosing protruding gallbladder lesions.