| 摘要: |
| 目的 探讨抗MAGE抗体偶联的载金纳米棒靶向纳米探针的制备和表征,并对纳米探针对体外恶性黑色素瘤细胞的靶向性进行了研究, 观察其体外光声成像效果，为其在后续生物医学应用如光热治疗等奠定坚实的基础。方法 采用双乳化法制备包裹金纳米棒(gold nanorod，GNR)及液态氟碳（PFH）的PLGA纳米粒(gold nanoparticle，GNP)。碳二亚胺法连接靶向恶性黑色素瘤的单克隆抗体(MAGE-Antibody)，制备高分子多功能靶向PLGA纳米分子探针,检测其一般物理特性、MAGE抗体与纳米粒的连接情况及其体外寻靶能力，体外观察光声成像效果。结果 成功制备的靶向载金纳米棒多功能纳米分子探针平均粒径为(336．40±27. 46) nm，Zeta电位(-4．34±4．9)mV；体外寻靶能力实验显示黑色素瘤B16细胞株周围有大量靶向纳米探针环绕。体外光声成像显示随金纳米粒含量增加，其光声信号逐渐增强。结论 MAGE-Antibody偶联金纳米棒纳米探针制备成功,对体外恶性黑色素瘤细胞具有较好的生物活性,并且细胞靶向性较好。体外光声有明显增强信号。可作为光声成像造影剂，进一步行肿瘤光热治疗。 |
| 关键词: 光声技术 金纳米棒 靶向纳米探针 恶性黑色素瘤 |
| DOI： |
| 投稿时间：2017-09-07修订日期：2017-09-22 |
| 基金项目:国家自然科学基金重大项目,国家自然科学基金项目（面上项目，重点项目，重大项目） |
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| Study on MAGE-Antibody targeting PLGA nanoparticle loaded with Gold nanorod and liquid perfluorocarbon for photoacoustic imaging in vitro |
| lixuelin,Wangzhigang |
| () |
| Abstract: |
| Objective To investigate the ability of targeting in vitro melanoma of nanoparticle probe loaded with Gold Nanorod and observe its consequence of photoacoustic imaging in vitro. Methods The polymeric multifunctional nanoparticles probe loaded with Gold Nanorod and liquid perfluorocarbon， and connected with MAGE-Antibody targeting melanoma was prepared in the use of double emulsion method and carbodiimide method．General physical property，the condition of MAGE-Antibody connected with nanoparticles and the ability of targeting of the probe were tested． The different concentration of nanoparticles was imaged by photoacoustic．The inhibiting effect of targeting nanoparticles on melanoma cells was evaluated in vitro． Results The targeting polymeric multifunctional nanoparticles probes loaded with Gold Nanorod were prepared successfully with average particle diameter of(336．4±27．46)nm and Zeta potential(-4．34±4．9)mV， Gold Nanorod particles dispersed on the shell of the probes．MAGE-Antibody was successfully connected with the surface of the nanoprobes． There were massive targeting nanoprobes surround the melanoma cell strains B16 in the targeting group in vitro．The photoacoustic signal of the nanoprobes enhanced with the increase of Gold Nanorod concentration in photoacoustic experiment in vitro． Conclusion The prepared polymeric multifunctional nanoparticles probe that was loaded with Gold Nanorod and connected with MAGE-Antibody targeting melanoma can be used as a photoacoustic contrast agent，which can inhibit the proliferation of melanoma by targeting photoacoustic therapy． |
| Key words: Photoacoustic imaging Gold nanorod Targeted nano probe Melanoma |
